| Mittera Group, Inc. | Manufacturing | $10,000,000 | $10,115,278 | 499 | $494.9M |  Research |
| Anderson Erickson Dairy Co. | Manufacturing | $6,299,577 | $6,345,486 | 331 | $116.8M | Research |
| Lutheran Services in Iowa INC | Health Care and Social Assistance | $4,964,519 | $4,996,346 | 500 | $81.0M | Research |
| Elder Corporation | Construction | $4,810,100 | $4,865,054 | 311 | $116.5M | Research |
| Loffredo Gardens, Inc. | Manufacturing | $4,809,613 | $4,864,825 | 469 | $165.5M | Research |
| Primary Health Care, Inc. | Health Care and Social Assistance | $4,789,500 | $4,845,268 | 363 | N/A | Research |
| Iowa Orthopaedic Center, P.c. | Health Care and Social Assistance | $4,462,300 | $4,513,403 | 313 | $98.2M | Research |
| Doll Distributing LLC | Wholesale Trade | $4,370,600 | $4,425,840 | 316 | $81.3M | Research |
| Seneca Companies, Inc. | Wholesale Trade | $4,016,373 | $4,063,788 | 288 | $74.1M | Research |
| Electrical Power Products Inc. | Manufacturing | $3,969,800 | $4,015,154 | 334 | $87.5M | Research |
| Visiting Nurse Services of Iowa | Health Care and Social Assistance | $3,642,400 | $3,683,714 | 357 | N/A | Research |
| Nyemaster Goode, P.c. | Professional, Scientific, and Technical Services | $3,574,393 | $0 | 161 | $39.1M | Research |
| Grand View University | Educational Services | $3,537,900 | $3,557,016 | 350 | N/A | Research |
| Barton Solvents, INC | Wholesale Trade | $3,434,100 | $3,473,616 | 194 | $49.9M | Research |
| Iowa Beverage Systems, Inc. | Wholesale Trade | $3,404,000 | $3,443,997 | 315 | $81.1M | Research |
| Graham Construction Company | Construction | $3,220,100 | $2,982,448 | 128 | $145.8M | Research |
| Orchard Place | Other Services (except Public Administration) | $3,181,800 | $3,217,977 | 355 | N/A | Research |
| Gl Dodge City, LLC | Retail Trade | $2,879,247 | $2,916,791 | 198 | $49.9M | Research |
| Millenium Rehab & Consulting Group Inc. | Health Care and Social Assistance | $2,866,400 | $2,899,069 | 306 | $82.6M | Research |
| Lifeserve Blood Center | Other Services (except Public Administration) | $2,796,100 | $2,827,891 | 272 | N/A | Research |
| Medical Oncology and Hermatology | Health Care and Social Assistance | $2,765,325 | $2,800,176 | 208 | $65.3M | Research |
| Scharnberg Holding Company | Manufacturing | $2,719,600 | $2,751,329 | 250 | $69.8M | Research |
| Charles Gabus Ford, Inc. | Retail Trade | $2,699,800 | $2,730,792 | 236 | $59.5M | Research |
| Davis, Brown, Koehn, Shors & Roberts, P.c. | Professional, Scientific, and Technical Services | $2,614,000 | $2,658,656 | 148 | $36.0M | Research |
| Storey Kenworthy Company | Retail Trade | $2,527,715 | $2,556,593 | 213 | $46.2M | Research |