| Woodward Communications, Inc. | Information | $6,500,000 | $6,613,438 | 500 | $84.0M |  Research |
| Dubuque Internal Medicine PC | Health Care and Social Assistance | $4,348,400 | $4,404,688 | 367 | $66.9M | Research |
| Loras College | Educational Services | $3,859,200 | $3,901,544 | 491 | N/A | Research |
| Hillcrest Family Services | Health Care and Social Assistance | $3,195,000 | $3,237,542 | 243 | N/A | Research |
| Hills & Dales Child Development Center | Health Care and Social Assistance | $2,974,600 | $3,010,047 | 317 | N/A | Research |
| Kendall Hunt Publishing Company | Information | $2,974,500 | $3,027,552 | 203 | $34.1M | Research |
| Clarke University of Dubuque, Iowa | Educational Services | $2,909,100 | $2,943,770 | 462 | N/A | Research |
| Parco Ltd. | Accommodation and Food Services | $2,839,000 | $2,871,806 | 250 | $44.4M | Research |
| Conlon Construction Co. | Construction | $2,835,300 | $2,868,300 | 202 | $230.1M | Research |
| Hartig Drug Company | Wholesale Trade | $2,541,100 | $2,570,534 | 386 | $99.4M | Research |
| Holy Family Catholic Schools | Educational Services | $2,514,700 | $2,543,619 | 425 | N/A | Research |
| Stonehill Franciscan Services | Health Care and Social Assistance | $2,455,400 | $2,483,978 | 336 | N/A | Research |
| Portzen Construction, Inc. | Construction | $2,336,400 | $2,363,413 | 113 | $128.7M | Research |
| Conlon Construction Company | Construction | $2,000,000 | $2,018,278 | 174 | $198.2M | Research |
| Woodward Communications Inc. | Professional, Scientific, and Technical Services | $2,000,000 | $2,022,795 | 299 | $33.5M | Research |
| Hillcrest Family Services | Health Care and Social Assistance | $2,000,000 | $2,017,753 | 300 | N/A | Research |
| Westmark Enterprises, Inc. | Construction | $1,918,500 | $1,940,155 | 135 | $41.8M | Research |
| Klauer Manufacturing Company | Manufacturing | $1,845,600 | $1,857,186 | 124 | $30.3M | Research |
| Martin Luther Home Corp | Health Care and Social Assistance | $1,812,300 | $1,824,634 | 304 | N/A | Research |
| United Clinical Laboratories INC | Health Care and Social Assistance | $1,674,700 | $0 | 167 | $38.7M | Research |
| Dubuque Barge and Fleeting Service Company | Construction | $1,388,100 | $1,382,790 | 60 | $18.6M | Research |
| Dubuque Barge and Fleeting Service Company | Construction | $1,366,823 | $1,375,923 | 60 | $18.6M | Research |
| Roc Taproom Inc. | Accommodation and Food Services | $1,302,000 | $1,319,179 | 210 | $37.3M | Research |
| Platinum Supplemental Insurance, INC | Finance and Insurance | $1,267,700 | $1,282,912 | 71 | $36.5M | Research |
| Faley Enterprises, Inc. | Wholesale Trade | $1,260,900 | $1,270,607 | 254 | $77.8M | Research |