| East Iowa Machine Company, LLC | Manufacturing | $2,195,900 | $2,221,702 | 154 | $37.6M |  Research |
| Simon's Trucking Inc. | Transportation and Warehousing | $1,810,700 | $1,827,952 | 149 | $30.0M | Research |
| Behnke Enterprises, Inc. | Manufacturing | $1,163,700 | $1,175,046 | 103 | $27.0M | Research |
| Lund Manufacturing Co., Inc. | Manufacturing | $506,800 | $512,699 | 61 | $16.0M | Research |
| Kluesner Construction INC | Construction | $477,400 | $481,047 | 42 | $14.3M | Research |
| Top Grade Excavating, INC | Construction | $421,900 | $425,148 | 22 | $6.8M | Research |
| Western Dubuque Biodiesel LLC | Wholesale Trade | $387,000 | $389,580 | 21 | $5.4M | Research |
| Western Dubuque Biodiesel LLC | Manufacturing | $368,900 | $370,939 | 24 | $23.8M | Research |
| Rauen Precision Machining INC | Manufacturing | $309,835 | $311,677 | 24 | $6.3M | Research |
| Rauen Precision Machining INC | Other Services (except Public Administration) | $301,900 | $303,737 | 24 | $5.2M | Research |
| Seton Catholic School | Educational Services | $290,100 | $291,510 | 49 | N/A | Research |
| Seton Catholic School | Educational Services | $281,000 | $282,920 | 48 | N/A | Research |
| Tri-Vet Associates, Inc. | Professional, Scientific, and Technical Services | $234,900 | $236,206 | 19 | <$5M | Research |
| Concrete Concepts INC | Construction | $176,772 | $177,794 | 14 | <$5M | Research |
| Concrete Concepts Inc. | Construction | $161,000 | $162,239 | 17 | <$5M | Research |
| Strief Farms Inc. | Agriculture, Forestry, Fishing and Hunting | $149,341 | $150,032 | 18 | <$5M | Research |
| J P Scherrman, Inc. | Wholesale Trade | $147,700 | $149,230 | 10 | <$5M | Research |
| Kluesner Sanitation, LLC | Administrative and Support and Waste Management | $135,100 | $136,128 | 14 | <$5M | Research |
| Farley Dental Center, P.c. | Health Care and Social Assistance | $128,700 | $129,495 | 11 | <$5M | Research |
| Strief Farms, Inc. | Agriculture, Forestry, Fishing and Hunting | $118,480 | $119,350 | 15 | <$5M | Research |
| Locher & Davis Plc | Professional, Scientific, and Technical Services | $118,400 | $120,150 | 18 | <$5M | Research |
| Triton Manufacturing Corporation | Wholesale Trade | $113,575 | $107,059 | 10 | <$5M | Research |
| Farley Dental Center P.c. | Health Care and Social Assistance | $104,246 | $104,834 | 11 | <$5M | Research |
| Precision Wire & Design Inc. | Manufacturing | $92,300 | $93,144 | 7 | <$5M | Research |
| Duschner Farms | Agriculture, Forestry, Fishing and Hunting | $89,990 | $90,474 | 18 | <$5M | Research |