| Mci, Lc | Administrative and Support and Waste Management | $7,134,900 | $7,215,960 | 500 | $94.6M |  Research |
| Goodwill Industries of the Heartland | Health Care and Social Assistance | $4,500,000 | $4,531,125 | 500 | $81.0M | Research |
| Mccomas-Lacina Construction Lc | Construction | $2,159,700 | $2,187,476 | 132 | $150.4M | Research |
| Mccomas-Lacina Construction Lc | Construction | $2,000,000 | $2,017,611 | 113 | $128.7M | Research |
| Billion Community, INC | Retail Trade | $1,760,000 | $1,790,262 | 161 | $40.6M | Research |
| The River Products Company | Mining, Quarrying, and Oil and Gas Extraction | $1,614,500 | $1,633,201 | 147 | $8.9M | Research |
| Christian Retirement Services, Inc. | Health Care and Social Assistance | $1,540,500 | $1,550,385 | 253 | N/A | Research |
| Dreusicke Holdings, Inc. | Real Estate and Rental and Leasing | $1,264,300 | $1,273,361 | 76 | $11.4M | Research |
| Steindler Orthopedic Clinic Plc | Health Care and Social Assistance | $1,249,692 | $1,254,482 | 88 | $27.6M | Research |
| New Pioneer's Cooperative Society | Retail Trade | $1,234,700 | $1,246,327 | 168 | $41.9M | Research |
| Knutson Construction Services Midwest, Inc. | Construction | $1,206,800 | $1,214,438 | 62 | $70.6M | Research |
| The Economy Advertising Company | Professional, Scientific, and Technical Services | $1,149,600 | $1,162,757 | 94 | $10.5M | Research |
| Prelude Behavior Services | Health Care and Social Assistance | $1,140,962 | $1,151,991 | 106 | N/A | Research |
| Prelude Behavioral Services | Other Services (except Public Administration) | $1,140,900 | $1,148,886 | 110 | N/A | Research |
| Autohaus Ltd | Retail Trade | $1,049,700 | $1,063,958 | 74 | $18.6M | Research |
| Steindler Orthopedic Clinic Plc | Health Care and Social Assistance | $1,035,800 | $1,421,152 | 73 | $22.9M | Research |
| Regina Inter-Parish Catholic Education Center | Educational Services | $1,003,900 | $1,011,680 | 108 | N/A | Research |
| Bread Garden of Iowa City L.c. | Accommodation and Food Services | $977,300 | $984,657 | 106 | $18.8M | Research |
| Reach for Your Potential, Inc. | Other Services (except Public Administration) | $950,000 | $957,943 | 207 | N/A | Research |
| Progressive Rehabilitation Associates, LLC | Health Care and Social Assistance | $937,200 | $942,953 | 98 | $26.5M | Research |
| Progressive Rehabilittation Associates LLC | Health Care and Social Assistance | $937,200 | $942,589 | 104 | $28.1M | Research |
| Maxwell Construction, Inc. | Construction | $924,700 | $936,233 | 46 | $15.7M | Research |
| Streb Construction Co., Inc. | Construction | $912,500 | $922,639 | 36 | $11.2M | Research |
| Billion H, Inc. | Retail Trade | $895,000 | $908,425 | 75 | $18.9M | Research |
| Mayor's Youth Empowerment Program | Other Services (except Public Administration) | $893,100 | $817,492 | 199 | N/A | Research |