| Greene County Medical Center | Health Care and Social Assistance | $2,457,800 | $2,488,034 | 296 | N/A |  Research |
| Wild Rose Jefferson, LLC | Arts, Entertainment, and Recreation | $1,212,600 | $1,225,623 | 137 | $19.2M | Research |
| Conner Athletic Products, Inc. | Manufacturing | $836,200 | $843,308 | 71 | $18.6M | Research |
| Conner Athletic Products, Inc. | Manufacturing | $573,500 | $842,216 | 68 | $17.8M | Research |
| Tlh Project Mangement, LLC | Real Estate and Rental and Leasing | $529,700 | $534,649 | 34 | $5.1M | Research |
| Greene County Redi-Mix Inc. | Manufacturing | $377,940 | $379,597 | 22 | $21.8M | Research |
| Greene County Redi-Mix, INC | Manufacturing | $377,900 | $380,840 | 22 | $21.8M | Research |
| Empire Ag Llc. | Agriculture, Forestry, Fishing and Hunting | $350,444 | $355,107 | 30 | <$5M | Research |
| Harrison Rv Inc. | Retail Trade | $125,771 | $126,250 | 10 | <$5M | Research |
| Greene County Motor Company | Retail Trade | $119,300 | $120,096 | 7 | <$5M | Research |
| Jefferson Hotel Group | Accommodation and Food Services | $97,828 | $98,268 | 22 | $6.9M | Research |
| Harrison Rv, Inc. | Retail Trade | $92,400 | $92,954 | 9 | <$5M | Research |
| Mcatee Tire Service, Inc. | Other Services (except Public Administration) | $80,000 | $80,827 | 7 | <$5M | Research |
| Jefferson Hotel Group, LLC | Accommodation and Food Services | $75,100 | $75,658 | 22 | $6.9M | Research |
| Greene County Early Learning Center | Health Care and Social Assistance | $73,700 | $74,164 | 18 | N/A | Research |
| Greene County Early Learning Center | Health Care and Social Assistance | $72,217 | $72,516 | 10 | N/A | Research |
| Durlam Electric, INC | Construction | $68,000 | $68,432 | 7 | <$5M | Research |
| Brooker & Company, Inc. | Professional, Scientific, and Technical Services | $67,200 | $0 | 4 | <$5M | Research |
| Jefferson Family Dentistry, PC | Health Care and Social Assistance | $66,320 | $67,073 | 7 | <$5M | Research |
| Jefferson Family Dentistry PC | Health Care and Social Assistance | $66,320 | $66,821 | 8 | <$5M | Research |
| Natural Wonders Learning Center LLC | Health Care and Social Assistance | $61,000 | $61,440 | 16 | <$5M | Research |
| Complete Insurance Ltd | Finance and Insurance | $60,500 | $60,873 | 6 | <$5M | Research |
| Anglemyer Pharmacies, LLC | Retail Trade | $56,000 | $56,629 | 6 | <$5M | Research |
| Natural Wonders Learning Center LLC | Health Care and Social Assistance | $54,913 | $55,244 | 20 | <$5M | Research |
| Sfs Ventures, Inc. | Other Services (except Public Administration) | $54,600 | $55,083 | 10 | <$5M | Research |