| Open Gates Business Development | Professional, Scientific, and Technical Services | $670,300 | $673,987 | 87 | $9.0M |  Research |
| Iowa Mennonite Benevolent Association | Health Care and Social Assistance | $635,000 | $638,528 | 133 | N/A | Research |
| Blake Hershberger Enterprises LLC | Construction | $628,500 | $632,009 | 37 | $8.6M | Research |
| Blake Hershberger Enterprises LLC | Construction | $608,872 | $611,899 | 52 | $12.0M | Research |
| Gingerich Well & Pump Service LLC | Construction | $455,015 | $458,011 | 37 | $11.5M | Research |
| Farmers Hen House Group, Inc. | Agriculture, Forestry, Fishing and Hunting | $451,000 | $452,817 | 58 | $9.5M | Research |
| Gingerich Well & Pump Service LLC | Construction | $446,600 | $451,376 | 37 | $13.9M | Research |
| Slabach Construction Co INC | Manufacturing | $247,900 | $249,491 | 19 | $5.3M | Research |
| Iowa Mennonite School | Educational Services | $243,300 | $244,645 | 37 | N/A | Research |
| Kalona Brewing Company | Accommodation and Food Services | $241,283 | $243,312 | 30 | $5.3M | Research |
| Kalona Tire, Inc. | Other Services (except Public Administration) | $241,100 | $243,303 | 30 | $5.9M | Research |
| The Appliance Barn Inc. | Retail Trade | $239,900 | $241,339 | 23 | $7.0M | Research |
| Kalona Co-Operative Telephone Company | Information | $239,267 | $240,503 | 19 | <$5M | Research |
| Foundations Unlimited, Inc. | Construction | $228,500 | $230,239 | 18 | <$5M | Research |
| Foundations Unlimited Inc. | Construction | $228,500 | $229,846 | 20 | <$5M | Research |
| Kuenster Heating & Air, LLC | Construction | $182,400 | $184,320 | 18 | $5.7M | Research |
| Kuenster Heating & Air LLC | Construction | $182,400 | $184,144 | 17 | $5.4M | Research |
| J & S Plumbing, Heating and Air Conditioning, Inc. | Construction | $162,800 | $164,053 | 16 | $5.1M | Research |
| Kalona Brewing Company | Manufacturing | $157,068 | $158,238 | 30 | $10.6M | Research |
| Slabaugh Trucking INC | Transportation and Warehousing | $154,700 | $155,718 | 16 | <$5M | Research |
| J & S Plumbing Heating and Air Conditioning Inc. | Construction | $153,462 | $154,737 | 16 | $5.1M | Research |
| King Bros Inc. | Transportation and Warehousing | $140,076 | $140,898 | 13 | <$5M | Research |
| Miller Hybrids Inc. | Wholesale Trade | $139,375 | $139,940 | 12 | <$5M | Research |
| Miller Hybrids, Inc. | Agriculture, Forestry, Fishing and Hunting | $133,700 | $134,721 | 12 | <$5M | Research |
| Chris Schlabach Construction Inc. | Construction | $119,850 | $120,674 | 10 | <$5M | Research |