| Schuster Co. | Transportation and Warehousing | $4,553,100 | $4,606,725 | 363 | $82.8M |  Research |
| City of Lemars, Iowa | Health Care and Social Assistance | $3,735,200 | $3,777,873 | 331 | N/A | Research |
| Nor-Am Cold Storage INC | Transportation and Warehousing | $1,629,200 | $1,643,093 | 164 | $41.6M | Research |
| Smithco Mfg., Inc. | Manufacturing | $1,452,500 | $1,469,094 | 119 | $31.2M | Research |
| Smithco Mfg. Inc. | Manufacturing | $1,423,729 | $1,434,222 | 110 | $28.8M | Research |
| Plymouth Life, Inc. | Real Estate and Rental and Leasing | $1,273,925 | $1,283,763 | 170 | N/A | Research |
| Plains Area Mental Health, Inc. | Health Care and Social Assistance | $1,182,200 | $1,191,395 | 123 | N/A | Research |
| Le Mars Truck and Trailer Inc. | Administrative and Support and Waste Management | $1,155,400 | $1,164,483 | 102 | $16.1M | Research |
| Anthony Pit and Lagoon Inc. | Transportation and Warehousing | $925,800 | $933,155 | 70 | $10.6M | Research |
| Nor-Am Ice and Cold LLC | Transportation and Warehousing | $873,900 | $882,372 | 96 | $24.4M | Research |
| Total Motors LLC | Retail Trade | $774,900 | $779,295 | 54 | $13.6M | Research |
| Gehlen Catholic School | Educational Services | $648,000 | $653,042 | 61 | N/A | Research |
| Mccormack Distributing Company, Inc. | Wholesale Trade | $637,300 | $642,062 | 40 | $12.3M | Research |
| Mccormack Distributing Company, Inc. | Wholesale Trade | $617,200 | $619,583 | 42 | $12.9M | Research |
| Iml Containers Iowa, Inc. | Wholesale Trade | $610,832 | $617,483 | 47 | $12.1M | Research |
| Four-Way Logistics Inc. | Manufacturing | $555,400 | $559,250 | 70 | $69.4M | Research |
| Nor-Am Logistics INC | Transportation and Warehousing | $550,600 | $555,020 | 64 | $16.2M | Research |
| Plymouth Dairy Farms, INC | Agriculture, Forestry, Fishing and Hunting | $535,100 | $538,853 | 58 | $9.5M | Research |
| Buy Rite Logistics, INC | Manufacturing | $447,700 | $452,030 | 63 | $62.5M | Research |
| Jensen Motors INC | Retail Trade | $408,503 | $410,764 | 38 | $9.6M | Research |
| Tritz Pallet, Inc. | Manufacturing | $382,000 | $384,941 | 40 | $39.7M | Research |
| Van's Sanitation, Inc. | Transportation and Warehousing | $363,400 | $365,819 | 38 | $9.5M | Research |
| Herbst Construction INC | Construction | $333,125 | $335,617 | 18 | $5.6M | Research |
| Herbst Construction, Inc. | Construction | $312,600 | $314,364 | 27 | $8.4M | Research |
| Brownmiller Leasing & Transportation, Inc. | Transportation and Warehousing | $254,200 | $255,979 | 25 | $5.0M | Research |