| Cornell College | Educational Services | $4,489,200 | $4,541,325 | 470 | N/A |  Research |
| Lynch Ford-Mt Vernon, Inc. | Retail Trade | $1,194,832 | $1,200,541 | 105 | $26.5M | Research |
| Lynch Ford-Mt Vernon, Inc. | Retail Trade | $1,186,500 | $1,193,817 | 92 | $23.2M | Research |
| US Nameplate Company, Inc. | Manufacturing | $576,400 | $579,650 | 51 | $50.6M | Research |
| Eastern Iowa Blinds, Inc. | Construction | $373,337 | $376,661 | 25 | <$5M | Research |
| Ability Physical Therapy P.c. | Health Care and Social Assistance | $313,680 | $315,205 | 35 | $9.4M | Research |
| Ability Physical Therapy P.c. | Health Care and Social Assistance | $308,300 | $310,732 | 38 | $10.3M | Research |
| D & G, Inc. | Retail Trade | $195,575 | $196,705 | 56 | $14.0M | Research |
| Poly Vinyl Roofing, Inc. | Construction | $164,800 | $165,807 | 19 | $6.3M | Research |
| Paa, LLC | Wholesale Trade | $154,300 | $132,649 | 15 | <$5M | Research |
| Mt Vernon Construction, INC | Construction | $154,000 | $155,219 | 16 | $5.5M | Research |
| Rocarek Enterprises, INC | Construction | $124,886 | $125,488 | 11 | <$5M | Research |
| Rocarek Enterprises, INC | Administrative and Support and Waste Management | $124,800 | $125,909 | 11 | <$5M | Research |
| Mt. Vernon Eye Clinic P.l.c. | Health Care and Social Assistance | $105,898 | $106,389 | 9 | <$5M | Research |
| Dawn M. Gaffney, D.d.s., L.l.c. | Health Care and Social Assistance | $102,300 | $102,972 | 9 | <$5M | Research |
| Mt. Vernon Eye Clinic, Plc | Health Care and Social Assistance | $95,200 | $95,882 | 9 | <$5M | Research |
| M & K Dust Control, Inc. | Transportation and Warehousing | $88,500 | $89,515 | 9 | <$5M | Research |
| Mount Vernon Family Dentistry PC | Health Care and Social Assistance | $86,100 | $86,647 | 7 | <$5M | Research |
| Pinnacle Investments, LLC | Accommodation and Food Services | $83,813 | $84,297 | 12 | <$5M | Research |
| I.c. Mill Associates, L.l.c. | Accommodation and Food Services | $74,600 | $10,484 | 16 | <$5M | Research |
| Neal's Water Conditioning Service Inc. | Utilities | $73,509 | $73,793 | 7 | N/A | Research |
| Koppenhaver & Associates, PC | Professional, Scientific, and Technical Services | $71,000 | $71,264 | 5 | <$5M | Research |
| Vg Stoner & Sons Corp | Agriculture, Forestry, Fishing and Hunting | $69,400 | $69,824 | 13 | <$5M | Research |
| Vg Stoner & Sons Corp | Agriculture, Forestry, Fishing and Hunting | $69,400 | $69,664 | 13 | <$5M | Research |
| Bennett Painting & Decorating INC | Construction | $61,928 | $62,187 | 6 | <$5M | Research |