| Story County Hospital | Health Care and Social Assistance | $2,454,402 | $2,481,469 | 199 | N/A |  Research |
| Gary W Clem, Inc. | Manufacturing | $2,402,704 | $2,426,666 | 158 | $41.4M | Research |
| Hertz Farm Management Inc. | Agriculture, Forestry, Fishing and Hunting | $1,979,166 | $1,989,007 | 210 | $10.6M | Research |
| Hertz Farm Management, Inc. | Agriculture, Forestry, Fishing and Hunting | $1,979,100 | $1,999,123 | 128 | $6.4M | Research |
| Gary W. Clem Inc. | Manufacturing | $1,900,000 | $1,919,312 | 168 | $44.0M | Research |
| 21st Century Rehab, P.c. | Health Care and Social Assistance | $1,154,159 | $1,160,956 | 93 | $25.1M | Research |
| Jcg Land Services, Inc. | Real Estate and Rental and Leasing | $1,066,609 | $1,081,161 | 64 | $11.7M | Research |
| Mid-States Millwright & Builders, Inc. | Construction | $608,735 | $617,407 | 46 | $52.4M | Research |
| Lincolnway Energy LLC | Manufacturing | $505,700 | $511,034 | 35 | $34.7M | Research |
| Iowa Insulation INC | Construction | $361,200 | $364,169 | 32 | $10.2M | Research |
| Mid-America Manufacturing, INC | Manufacturing | $352,453 | $354,944 | 33 | $8.6M | Research |
| Paragon International, Inc. | Manufacturing | $348,360 | $351,013 | 41 | $10.7M | Research |
| Paragon International, Inc. | Manufacturing | $348,359 | $350,497 | 32 | $8.4M | Research |
| On-Track Construction LLC | Construction | $320,000 | $321,698 | 28 | $8.5M | Research |
| Mid-States Material Handling & Fabrication, Inc. | Manufacturing | $260,919 | $264,636 | 24 | $5.9M | Research |
| Frontline Bioenergy, LLC | Professional, Scientific, and Technical Services | $200,885 | $201,958 | 16 | <$5M | Research |
| Midwest Insurance Corporation | Finance and Insurance | $187,200 | $188,245 | 14 | $7.2M | Research |
| Equipoise Accounting & Finance | Professional, Scientific, and Technical Services | $152,377 | $153,216 | 12 | <$5M | Research |
| Hetzler & Rhodes Concrete Construction, Inc. | Construction | $145,033 | $146,201 | 13 | <$5M | Research |
| Stephenson Dental, Inc. | Health Care and Social Assistance | $139,343 | $140,222 | 13 | <$5M | Research |
| Agsource Seeds INC | Agriculture, Forestry, Fishing and Hunting | $130,800 | $131,697 | 9 | <$5M | Research |
| Dave Jensen Trucking LLC | Transportation and Warehousing | $129,744 | $130,373 | 7 | <$5M | Research |
| Dave Jensen Trucking LLC | Transportation and Warehousing | $129,744 | $130,430 | 7 | <$5M | Research |
| Agsource Seeds INC | Agriculture, Forestry, Fishing and Hunting | $124,593 | $124,870 | 33 | <$5M | Research |
| Longview Farms | Agriculture, Forestry, Fishing and Hunting | $121,597 | $110,845 | 12 | <$5M | Research |