| Mid-State Milling, Inc. | Manufacturing | $723,400 | $728,203 | 56 | $19.8M |  Research |
| Marshall Ridge Farms, LLC | Wholesale Trade | $454,700 | $457,630 | 47 | $12.1M | Research |
| Engineered Equipment Solutions Inc. | Professional, Scientific, and Technical Services | $127,300 | $127,735 | 6 | <$5M | Research |
| Engineered Equipment Solutions, Inc. | Professional, Scientific, and Technical Services | $114,900 | $115,583 | 6 | <$5M | Research |
| Central Millwright Service LLC | Construction | $100,215 | $100,841 | 8 | <$5M | Research |
| Central Millwright Services, LLC | Construction | $100,214 | $101,112 | 8 | <$5M | Research |
| Edler Brothers | Agriculture, Forestry, Fishing and Hunting | $95,700 | $96,553 | 5 | <$5M | Research |
| Edler Management Services LLP | Agriculture, Forestry, Fishing and Hunting | $76,075 | $76,753 | 6 | <$5M | Research |
| Brian's Collision Clinic LLC | Other Services (except Public Administration) | $73,075 | $65,324 | 5 | <$5M | Research |
| Iowa Meat Company, Inc. | Retail Trade | $57,400 | $57,746 | 10 | <$5M | Research |
| Johnson Livestock LLC | Transportation and Warehousing | $55,335 | $55,616 | 4 | <$5M | Research |
| Benson Hill Farms Inc. | Agriculture, Forestry, Fishing and Hunting | $52,962 | $53,265 | 9 | <$5M | Research |
| Thomas Farms Custom Ag, LLC | Wholesale Trade | $51,300 | $51,760 | 24 | $6.2M | Research |
| Jjj Edler Partnership | Wholesale Trade | $50,900 | $51,180 | 5 | <$5M | Research |
| Eric Edler | Agriculture, Forestry, Fishing and Hunting | $49,165 | $49,705 | 4 | <$5M | Research |
| Eckhart Bros | Agriculture, Forestry, Fishing and Hunting | $41,666 | $41,807 | 2 | <$5M | Research |
| David L Eggers | Agriculture, Forestry, Fishing and Hunting | $41,600 | $41,835 | 2 | <$5M | Research |
| West Marshall Golf Course Association | Arts, Entertainment, and Recreation | $38,000 | $38,207 | 23 | <$5M | Research |
| Indepenent Ag Services LLC | Professional, Scientific, and Technical Services | $32,977 | $33,093 | 3 | <$5M | Research |
| Eckhardt Enterprises | Finance and Insurance | $32,900 | $33,084 | 5 | <$5M | Research |
| St. Andrews Grill & Bar | Accommodation and Food Services | $32,500 | $32,654 | 13 | <$5M | Research |
| Independent Ag Services LLC | Wholesale Trade | $31,700 | $31,936 | 4 | <$5M | Research |
| Water Technology Group Midwest Inc. | Professional, Scientific, and Technical Services | $28,400 | $28,497 | 3 | <$5M | Research |
| Cy-Hawk Insurance, Inc. | Finance and Insurance | $28,200 | $17,667 | 2 | <$5M | Research |
| Dan L. Freiberg | Agriculture, Forestry, Fishing and Hunting | $27,082 | $27,244 | 1 | <$5M | Research |