| Washington County Hospital | Health Care and Social Assistance | $4,288,400 | $3,796,524 | 336 | $66.7M |  Research |
| Brenneman Pork, Lllp | Agriculture, Forestry, Fishing and Hunting | $1,812,700 | $1,831,834 | 146 | $23.9M | Research |
| Bazooka Farmstar LLC | Agriculture, Forestry, Fishing and Hunting | $1,243,500 | $1,258,940 | 106 | $5.3M | Research |
| Iowa Bridge & Culvert, L.c. | Construction | $1,063,974 | $1,070,831 | 75 | $23.2M | Research |
| Delong Construction INC | Construction | $1,028,325 | $1,036,095 | 88 | $27.3M | Research |
| Delong Construction, Inc. | Construction | $971,200 | $976,757 | 88 | $100.3M | Research |
| Engineered Building Design, Lc | Manufacturing | $930,000 | $937,983 | 82 | $81.3M | Research |
| Jerome W. Vittteoe Pork, Ltd. | Agriculture, Forestry, Fishing and Hunting | $881,800 | $885,595 | 79 | $12.9M | Research |
| Engineered Building Design L.c. | Manufacturing | $838,050 | $845,476 | 82 | $81.3M | Research |
| The United Presbyterian Home of Washington, Iowa | Health Care and Social Assistance | $751,700 | $755,751 | 109 | N/A | Research |
| Wildhawk Investments, LLC | Construction | $632,424 | $344,092 | 119 | $30.1M | Research |
| Premier 1 Supplies LLC | Agriculture, Forestry, Fishing and Hunting | $624,200 | $629,090 | 57 | <$5M | Research |
| Smartscripts Holdings, LLC | Retail Trade | $620,900 | $628,725 | 42 | $7.3M | Research |
| Greiner Buildings, Inc. | Construction | $509,400 | $514,848 | 44 | $50.1M | Research |
| Washington Nursing and Rehabilitation Center, LLC | Health Care and Social Assistance | $443,825 | $448,750 | 61 | $10.4M | Research |
| Iowa Renewable Energy LLC | Manufacturing | $381,385 | $382,942 | 26 | $25.8M | Research |
| Iowa Renewable Energy, LLC | Manufacturing | $381,300 | $383,344 | 28 | $27.8M | Research |
| Cornerstone Excavating Inc. | Construction | $305,384 | $306,759 | 19 | $5.9M | Research |
| Cornerstone Excavating Inc. | Construction | $294,900 | $297,398 | 17 | $6.4M | Research |
| Wcdc Inc. | Health Care and Social Assistance | $291,545 | $293,400 | 47 | N/A | Research |
| Wcdc, Inc. | Health Care and Social Assistance | $291,500 | $293,775 | 54 | N/A | Research |
| Pharma340, LLC | Retail Trade | $268,949 | $271,041 | 30 | $5.2M | Research |
| Young Mens Christian Association of Washington Iowa | Other Services (except Public Administration) | $227,772 | $228,987 | 93 | N/A | Research |
| Young Men's Christian Association of Washington, Iowa | Arts, Entertainment, and Recreation | $227,700 | $229,629 | 100 | N/A | Research |
| Sitler's Supplies, Inc. | Wholesale Trade | $201,600 | $203,196 | 14 | <$5M | Research |