| Access Technologies, Inc. | Professional, Scientific, and Technical Services | $3,229,219 | $3,254,612 | 219 | $45.3M |  Research |
| Gilcrest Jewett Lumber Company | Wholesale Trade | $3,125,780 | $3,149,759 | 318 | $97.4M | Research |
| 1st Interiors, INC | Construction | $2,610,000 | $2,640,033 | 139 | $35.1M | Research |
| Rmh Systems, Inc. | Wholesale Trade | $1,837,300 | $1,847,317 | 97 | $29.7M | Research |
| Rmh Systems Inc. | Manufacturing | $1,820,785 | $1,831,361 | 101 | $28.2M | Research |
| Ed Stivers Ford | Retail Trade | $1,208,300 | $1,216,322 | 143 | $36.0M | Research |
| Express Logistics, Inc. | Transportation and Warehousing | $1,190,100 | $1,202,555 | 77 | $29.8M | Research |
| Shottenkirk Waukee, INC | Retail Trade | $1,069,600 | $1,081,878 | 101 | $25.5M | Research |
| Rj Lawn Service Inc. | Administrative and Support and Waste Management | $575,555 | $578,851 | 66 | $14.3M | Research |
| Deery Brothers of Adel, Inc. | Retail Trade | $568,200 | $571,376 | 50 | $12.6M | Research |
| Leafguard of Central Iowa, Inc. | Construction | $567,000 | $571,489 | 48 | $11.1M | Research |
| Rj Lawn Service, INC | Administrative and Support and Waste Management | $565,500 | $568,924 | 65 | $14.1M | Research |
| Myriad Advisor Solutions Inc. | Professional, Scientific, and Technical Services | $535,577 | $0 | 34 | <$5M | Research |
| Webster Electric, Inc. | Construction | $460,300 | $464,059 | 55 | $18.1M | Research |
| Rtl Delivery Lc | Transportation and Warehousing | $405,837 | $407,872 | 98 | $26.9M | Research |
| Central Standard Waukee LLC | Accommodation and Food Services | $372,814 | $375,030 | 64 | $7.8M | Research |
| Signature Health Care LLC | Health Care and Social Assistance | $344,500 | $390,240 | 75 | $12.2M | Research |
| Baumgart Home Care, Inc. | Health Care and Social Assistance | $337,100 | $339,338 | 105 | $18.0M | Research |
| Genesis Homes INC | Construction | $282,587 | $285,661 | 15 | <$5M | Research |
| Central Standard Waukee, LLC | Accommodation and Food Services | $266,296 | $268,508 | 64 | $7.8M | Research |
| System Dynamics, Inc. | Construction | $251,600 | $253,689 | 18 | $5.7M | Research |
| Websolv Computing, Inc. | Professional, Scientific, and Technical Services | $249,000 | $231,022 | 15 | <$5M | Research |
| Miller, Fidler & Hinke Insurance Agency, Inc. | Finance and Insurance | $233,500 | $235,426 | 14 | $7.2M | Research |
| Mid Iowa Electric INC | Other Services (except Public Administration) | $230,000 | $231,789 | 27 | $5.3M | Research |
| Websolv Computing Inc. | Professional, Scientific, and Technical Services | $229,600 | $230,461 | 15 | <$5M | Research |