| Hamilton County Public Hospital | Health Care and Social Assistance | $3,172,700 | $3,207,122 | 211 | $41.9M |  Research |
| Vantec, INC | Manufacturing | $2,053,300 | $1,889,487 | 196 | $194.4M | Research |
| Webster City Custom Meats, Inc. | Manufacturing | $1,516,500 | $1,527,552 | 129 | $45.5M | Research |
| Kastim Corp | Accommodation and Food Services | $1,295,100 | $1,308,441 | 195 | $34.6M | Research |
| Tasler Pallet & Lumber, Inc. | Manufacturing | $876,793 | $885,171 | 84 | $83.3M | Research |
| Mary Ann's Speciality Foods, Inc. | Manufacturing | $785,037 | $792,626 | 132 | $46.6M | Research |
| United Cooperative | Wholesale Trade | $705,975 | $710,642 | 62 | $16.0M | Research |
| Seneca Foundry, Inc. | Manufacturing | $555,570 | $558,533 | 38 | $9.9M | Research |
| Seneca Foundry INC | Manufacturing | $555,570 | $558,008 | 38 | $9.9M | Research |
| Mertz Engineering Co | Manufacturing | $487,820 | $490,480 | 48 | $11.7M | Research |
| Crestview Nursing and Rehabilitation, LLC | Health Care and Social Assistance | $470,420 | $474,067 | 88 | $15.0M | Research |
| North Star Ag Construction INC | Construction | $330,675 | $0 | 26 | $29.6M | Research |
| Jn Peterson & Sons, INC | Construction | $278,700 | $281,388 | 27 | $30.8M | Research |
| The Trash Man, LLC | Administrative and Support and Waste Management | $265,500 | $266,795 | 24 | <$5M | Research |
| Tony's Tire Service, Inc. | Transportation and Warehousing | $231,880 | $233,806 | 45 | <$5M | Research |
| Central Iowa Recovery | Health Care and Social Assistance | $172,900 | $164,470 | 18 | N/A | Research |
| North Star Ag Construction, INC | Construction | $162,500 | $0 | 17 | $5.2M | Research |
| Gourley Brothers Lc | Agriculture, Forestry, Fishing and Hunting | $161,987 | $163,013 | 17 | <$5M | Research |
| Stein Heating and Cooling Inc. | Construction | $157,600 | $157,437 | 14 | <$5M | Research |
| Stein Heating and Cooling INC | Construction | $157,600 | $158,922 | 15 | <$5M | Research |
| K & H Corporation | Construction | $156,800 | $158,063 | 13 | <$5M | Research |
| St. Thomas Aquinas Church Webster City, Iowa | Other Services (except Public Administration) | $148,480 | $149,684 | 19 | N/A | Research |
| St Thomas Aquinas Church Webster City Iowa | Other Services (except Public Administration) | $148,480 | $149,082 | 19 | N/A | Research |
| Casady Bros. Implement Inc. | Wholesale Trade | $134,200 | $123,380 | 11 | <$5M | Research |
| Life & Health Care, Inc. | Health Care and Social Assistance | $126,039 | $127,068 | 28 | <$5M | Research |