| W. W. Transport, Inc. | Transportation and Warehousing | $6,299,520 | $6,372,314 | 451 | $90.9M |  Research |
| Big River Resources West Burlington, LLC | Manufacturing | $3,657,300 | $2,854,238 | 202 | $200.4M | Research |
| Big River Resources West Burlington LLC | Manufacturing | $2,000,000 | $2,007,111 | 202 | $200.4M | Research |
| Borghi USA Inc. Oilhydraulic Conduits | Manufacturing | $1,427,440 | $1,213,367 | 122 | $29.8M | Research |
| Kpi Concepts LLC | Manufacturing | $1,421,400 | $1,434,943 | 163 | $161.7M | Research |
| Deery Brothers, Inc. | Retail Trade | $1,112,200 | $1,118,416 | 79 | $19.9M | Research |
| Burlington Stage Lines, Ltd | Transportation and Warehousing | $1,079,230 | $1,090,742 | 111 | $42.7M | Research |
| Burlington Stage Lines Ltd | Transportation and Warehousing | $1,079,230 | $1,086,455 | 76 | $29.2M | Research |
| Shottenkirk West Burlington, INC | Retail Trade | $665,500 | $673,140 | 72 | $18.1M | Research |
| Standard of Beaverdale, Inc. | Retail Trade | $323,120 | $326,091 | 38 | $7.8M | Research |
| Shottenkirk Automotive Group, INC | Retail Trade | $321,400 | $325,081 | 32 | $8.1M | Research |
| Valley Distribution Corp. | Wholesale Trade | $316,985 | $318,711 | 25 | $6.4M | Research |
| Deery Brothers Hm, Inc. | Retail Trade | $312,500 | $314,247 | 20 | $5.0M | Research |
| Four Seasons Excavation & Landscaping INC | Administrative and Support and Waste Management | $292,672 | $294,818 | 26 | $5.6M | Research |
| Four Seasons Excavation, INC | Administrative and Support and Waste Management | $258,000 | $261,483 | 25 | $5.4M | Research |
| Burlington Pediatric Association, P.c. | Health Care and Social Assistance | $251,000 | $252,417 | 19 | $6.0M | Research |
| Kinetic Cutting Systems, Inc. | Wholesale Trade | $237,900 | $240,892 | 12 | <$5M | Research |
| Burlington Neurology and Sleep Clinic Pplc | Health Care and Social Assistance | $233,600 | $235,449 | 24 | $7.5M | Research |
| Burlington Neurology & Sleep Clinic Plc | Health Care and Social Assistance | $233,600 | $235,774 | 25 | $7.8M | Research |
| Bi-State Contracting, Inc. | Construction | $222,830 | $224,203 | 12 | $13.7M | Research |
| Bi-State Contracting, Inc. | Construction | $222,830 | $223,977 | 12 | $13.7M | Research |
| Tck Concrete | Construction | $181,800 | $183,020 | 12 | <$5M | Research |
| Tck Concrete LLC | Construction | $170,400 | $171,362 | 10 | <$5M | Research |
| Broadway Grille Inc. | Accommodation and Food Services | $145,800 | $146,863 | 29 | $5.1M | Research |
| Michael Mathews DDS P.c. | Health Care and Social Assistance | $145,276 | $145,756 | 15 | <$5M | Research |