| Ita Group, Inc. | Accommodation and Food Services | $10,000,000 | $10,113,151 | 500 | $156.3M |  Research |
| Palmer Companies, Inc. | Administrative and Support and Waste Management | $5,651,725 | $5,736,578 | 75 | $11.8M | Research |
| E-Giants Technologies, LLC | Professional, Scientific, and Technical Services | $4,726,504 | $4,779,940 | 282 | $58.3M | Research |
| Des Moines Orthopaedic Surgeons PC | Health Care and Social Assistance | $4,485,600 | $4,538,057 | 250 | $78.5M | Research |
| Hubbell Realty Company & Subsidiaries | Construction | $4,175,000 | $4,208,632 | 248 | $75.3M | Research |
| Bsb Design INC | Professional, Scientific, and Technical Services | $3,603,300 | $2,886,367 | 168 | $52.1M | Research |
| Arona Corporation | Retail Trade | $3,352,900 | $3,392,017 | 500 | $90.6M | Research |
| All-State Industries, Inc. | Manufacturing | $3,012,200 | $3,047,426 | 266 | $263.8M | Research |
| Hirsh Industries, LLC | Manufacturing | $2,833,200 | $2,866,034 | 241 | $63.1M | Research |
| Iowa Home Care, LLC | Health Care and Social Assistance | $2,559,977 | $2,589,084 | 254 | $43.5M | Research |
| The Athletic Clubs by Tru Fit, LLC | Arts, Entertainment, and Recreation | $2,513,200 | $2,542,660 | 500 | $70.0M | Research |
| Lutheran Church of Hope | Other Services (except Public Administration) | $2,203,600 | $2,228,896 | 155 | N/A | Research |
| Dowling College | Educational Services | $2,060,227 | $2,083,877 | 240 | N/A | Research |
| Ivy Lane Corporation | Other Services (except Public Administration) | $2,021,700 | $1,924,901 | 290 | $56.8M | Research |
| Strategic America Inc. | Professional, Scientific, and Technical Services | $1,992,780 | $2,006,921 | 117 | $13.1M | Research |
| Strategic America INC | Professional, Scientific, and Technical Services | $1,992,700 | $2,011,481 | 117 | $13.1M | Research |
| Link Associates | Other Services (except Public Administration) | $1,960,600 | $1,979,239 | 291 | $55.6M | Research |
| Associated Anesthesiologists, P.c. | Health Care and Social Assistance | $1,910,600 | $1,931,643 | 63 | $19.8M | Research |
| Blue Stone Therapy, Inc. | Health Care and Social Assistance | $1,800,000 | $1,816,550 | 250 | $67.5M | Research |
| Grx Holdings LLC | Retail Trade | $1,761,079 | $1,773,015 | 239 | $41.7M | Research |
| Iec Hotel Corporation | Accommodation and Food Services | $1,735,426 | $1,749,167 | 120 | N/A | Research |
| Quality Consulting INC | Professional, Scientific, and Technical Services | $1,715,000 | $1,730,006 | 108 | $22.3M | Research |
| Innovative Injection Technologies Inc. | Manufacturing | $1,689,100 | $1,696,551 | 174 | $172.6M | Research |
| Wheeler Consolidated, Inc. | Wholesale Trade | $1,670,622 | $1,680,966 | 106 | $32.5M | Research |
| Corell Contractor INC | Construction | $1,620,900 | $1,536,995 | 86 | $26.7M | Research |