| V-Soft Consulting Group, Inc. | Professional, Scientific, and Technical Services | $10,000,000 | $10,114,795 | 500 | $103.4M |  Research |
| Cumberland Contura LLC | Mining, Quarrying, and Oil and Gas Extraction | $10,000,000 | $10,079,444 | 500 | $30.3M | Research |
| Bramco, Inc. | Wholesale Trade | $9,088,572 | $9,065,468 | 488 | $149.5M | Research |
| Presbyterian Church USA a Corp | Other Services (except Public Administration) | $8,850,500 | $8,952,772 | 500 | N/A | Research |
| Trilogy Management Services, LLC | Professional, Scientific, and Technical Services | $7,663,510 | $0 | 467 | $148.6M | Research |
| National Tobacco Company LP | Retail Trade | $7,485,000 | $7,594,917 | 455 | $71.0M | Research |
| Creation Gardens INC | Wholesale Trade | $7,390,700 | $7,303,482 | 421 | $108.4M | Research |
| Isco Industries, Inc. | Manufacturing | $7,200,500 | $7,284,906 | 487 | $483.0M | Research |
| Rudd Equipment Company Inc. | Wholesale Trade | $6,866,000 | $6,945,810 | 343 | $105.1M | Research |
| Restaurant Supply Chain Solutions, LLC | Accommodation and Food Services | $6,422,100 | $3,261,640 | 150 | $26.6M | Research |
| Kentuckiana Curb Company, Inc. | Manufacturing | $6,350,800 | $6,431,773 | 500 | $122.2M | Research |
| Home of the Innocents INC | Health Care and Social Assistance | $6,264,900 | $5,619,963 | 500 | N/A | Research |
| Mcm Cpas & Advisors LLP | Professional, Scientific, and Technical Services | $5,871,900 | $5,946,224 | 363 | $115.5M | Research |
| Ikeyless LLC | Administrative and Support and Waste Management | $5,833,500 | $5,915,007 | 294 | $43.1M | Research |
| Chemical & Industrial Engineering, Inc. | Professional, Scientific, and Technical Services | $5,502,905 | $5,566,720 | 252 | $31.1M | Research |
| Family Health Center, Inc. | Health Care and Social Assistance | $5,448,500 | $5,207,150 | 381 | N/A | Research |
| W. Frank Harshaw & Associates, INC | Wholesale Trade | $5,446,200 | $5,503,688 | 282 | $86.4M | Research |
| US Worldmeds LLC | Manufacturing | $5,204,000 | $0 | 209 | $207.3M | Research |
| The Young Men's Christian Association of Greater Louisville | Other Services (except Public Administration) | $5,164,700 | $5,215,917 | 123 | $23.5M | Research |
| The Broadhurst Group INC | Health Care and Social Assistance | $5,047,250 | $5,105,190 | 500 | N/A | Research |
| M & M Cartage Co., Inc. | Transportation and Warehousing | $5,000,000 | $5,529,831 | 400 | $60.5M | Research |
| Labor Works International, LLC | Administrative and Support and Waste Management | $4,963,100 | $5,021,297 | 284 | $44.7M | Research |
| Louisville Paving Company, Inc. | Construction | $4,952,400 | $5,009,794 | 215 | $66.6M | Research |
| Rainbow Design Services INC | Professional, Scientific, and Technical Services | $4,933,700 | $5,003,183 | 489 | $101.1M | Research |
| Up Properties Ii, LLC | Accommodation and Food Services | $4,909,700 | $4,966,330 | 500 | $88.8M | Research |