| J.r.s. Restaurant Corporation | Accommodation and Food Services | $2,297,328 | $2,323,825 | 500 | $88.8M |  Research |
| Middlesboro Coca-Cola Bottling Works, Inc. | Manufacturing | $2,095,215 | $2,118,612 | 230 | $81.2M | Research |
| Vaughn & Melton Consulting Engineers Inc. | Professional, Scientific, and Technical Services | $2,000,000 | $2,011,616 | 212 | $26.1M | Research |
| Heartland Inc. | Management of Companies and Enterprises | $1,038,481 | $1,044,596 | 260 | $42.7M | Research |
| Heartland, Inc. | Management of Companies and Enterprises | $1,038,480 | $1,045,317 | 290 | $47.7M | Research |
| Tennco INC | Mining, Quarrying, and Oil and Gas Extraction | $1,035,800 | $1,041,447 | 88 | $5.3M | Research |
| Tennco INC | Mining, Quarrying, and Oil and Gas Extraction | $856,900 | $672,080 | 80 | <$5M | Research |
| J.r. Hoe & Sons, Inc. | Manufacturing | $718,017 | $721,986 | 62 | $15.2M | Research |
| Vaughn & Melton Consulting Engineers, Inc. | Professional, Scientific, and Technical Services | $700,000 | $704,200 | 246 | $30.3M | Research |
| Tim Short Chrysler of Middlesboro | Retail Trade | $676,200 | $684,202 | 103 | $26.0M | Research |
| Corbin Restaurants INC | Accommodation and Food Services | $476,672 | $480,644 | 188 | $33.4M | Research |
| Combs Group LLC | Mining, Quarrying, and Oil and Gas Extraction | $302,635 | $304,863 | 24 | <$5M | Research |
| Combs Equipment Group, LLC | Manufacturing | $301,500 | $303,142 | 26 | $6.4M | Research |
| Green Construction Company, Inc. | Construction | $159,163 | $160,104 | 15 | <$5M | Research |
| Cumberland Ford Motors Inc. | Retail Trade | $155,216 | $156,122 | 18 | <$5M | Research |
| Cumberland Ford Motors Inc. | Retail Trade | $151,188 | $152,205 | 19 | <$5M | Research |
| Shoffner & Mars LLC | Accommodation and Food Services | $148,307 | $149,102 | 37 | $11.6M | Research |
| Bell Concrete Industries INC | Manufacturing | $133,853 | $134,637 | 17 | $16.9M | Research |
| Shake and Bake Motorsports, LLC | Arts, Entertainment, and Recreation | $133,000 | $134,574 | 23 | <$5M | Research |
| Sm Hospitality LLC | Accommodation and Food Services | $128,495 | $129,152 | 29 | $9.1M | Research |
| Golf Preservations, Inc. | Construction | $127,879 | $128,675 | 16 | <$5M | Research |
| Dubin Orthopaedic Centre P.s.c. | Health Care and Social Assistance | $117,618 | $118,295 | 16 | $5.0M | Research |
| Dubin Orthopaedic Centre, Psc | Health Care and Social Assistance | $110,497 | $111,159 | 15 | <$5M | Research |
| Shoffner & Mars LLC | Accommodation and Food Services | $110,347 | $111,263 | 36 | $11.3M | Research |
| Solid Steel Solutions, Inc. | Manufacturing | $108,005 | $109,511 | 20 | $5.2M | Research |