| Castlen Steel LLC | Manufacturing | $1,179,325 | $1,184,430 | 99 | $24.2M | AI Deep Dive |
| Consolidated Mechanical Inc. | Construction | $1,133,700 | $1,142,707 | 60 | $19.0M | AI Deep Dive |
| De Am Ron Building Systems LLC | Manufacturing | $1,001,600 | $1,001,127 | 105 | $104.1M | AI Deep Dive |
| Castlen Steel, LLC | Manufacturing | $999,274 | $1,005,242 | 92 | $24.1M | AI Deep Dive |
| Don Moore Chevrolet-Cadillac, Inc. | Retail Trade | $960,600 | $967,724 | 96 | $24.2M | AI Deep Dive |
| Owensboro Ford Center Inc. | Retail Trade | $959,368 | $967,201 | 75 | $18.9M | AI Deep Dive |
| Owensboro Ford Center, Inc. | Retail Trade | $928,458 | $935,555 | 75 | $18.9M | AI Deep Dive |
| Ernie Davis & Sons Mechanical, Inc. | Construction | $897,068 | $903,384 | 87 | $27.5M | AI Deep Dive |
| Sun Windows, Inc. | Manufacturing | $875,911 | $880,830 | 108 | $107.1M | AI Deep Dive |
| Consolidated Mechanical Inc. | Construction | $859,569 | $861,877 | 117 | $37.0M | AI Deep Dive |
| Orthopaedics & Sports Medicine Owensboro, P.s.c. | Health Care and Social Assistance | $855,472 | $864,097 | 76 | $23.9M | AI Deep Dive |
| Davco Homes, Inc. | Health Care and Social Assistance | $843,090 | $849,858 | 183 | $31.1M | AI Deep Dive |
| Owensboro Dermatology Associates, Psc | Health Care and Social Assistance | $841,800 | $846,851 | 63 | $19.8M | AI Deep Dive |
| Moonlite Bar-B-Que Inn Inc. | Accommodation and Food Services | $808,000 | $815,039 | 213 | $37.8M | AI Deep Dive |
| Vanguard Utility Service | Construction | $788,920 | $794,151 | 87 | $27.0M | AI Deep Dive |
| Hospice and Pallative Care | Health Care and Social Assistance | $773,300 | $786,747 | 80 | $13.7M | AI Deep Dive |
| Roman Catholic Diocese of Owensboro | Other Services (except Public Administration) | $754,595 | $760,094 | 47 | N/A | AI Deep Dive |
| Roman Catholic Diocese of Owensboro | Other Services (except Public Administration) | $754,595 | $758,647 | 47 | N/A | AI Deep Dive |
| Omico Employee Stock Ownership Plan and Trust | Manufacturing | $745,312 | $752,799 | 91 | $90.3M | AI Deep Dive |
| Devoted Senior Care LLC | Health Care and Social Assistance | $735,600 | $740,316 | 225 | $38.3M | AI Deep Dive |
| Glenn Enterprises, Inc. | Accommodation and Food Services | $721,500 | $724,521 | 60 | $18.8M | AI Deep Dive |
| Omico Inc. | Manufacturing | $718,930 | $723,333 | 80 | $79.3M | AI Deep Dive |
| Glenn Enterprises Inc. | Administrative and Support and Waste Management | $718,691 | $725,366 | 57 | $10.8M | AI Deep Dive |
| The Malcolm Bryant Corporation | Accommodation and Food Services | $708,547 | $717,787 | 119 | $37.2M | AI Deep Dive |
| Integrity Testing & Inspection, Incorporated | Professional, Scientific, and Technical Services | $662,200 | $667,080 | 48 | $9.7M | AI Deep Dive |