| Acme Plumbing & Heating Company, Inc. | Construction | $324,322 | $326,623 | 34 | $10.8M | AI Deep Dive |
| Scott Baird Plumbing & Heating Co., INC | Construction | $322,160 | $324,221 | 33 | $10.4M | AI Deep Dive |
| Bc Services LLC | Real Estate and Rental and Leasing | $320,800 | $323,723 | 24 | <$5M | AI Deep Dive |
| Ken's Carpets Unlimited, Inc. | Retail Trade | $317,100 | $321,522 | 34 | $6.2M | AI Deep Dive |
| Steve Jones Motors LLC | Retail Trade | $310,900 | $301,931 | 30 | $7.6M | AI Deep Dive |
| Owensboro Rv, Inc. | Retail Trade | $309,887 | $312,237 | 20 | $5.2M | AI Deep Dive |
| Neel Restaurants, LLC | Accommodation and Food Services | $307,400 | $309,671 | 50 | $8.9M | AI Deep Dive |
| Mechanical Consultants, Incorporated | Construction | $304,038 | $307,568 | 25 | $7.9M | AI Deep Dive |
| The Women's Pavilion | Health Care and Social Assistance | $296,782 | $298,967 | 24 | $7.5M | AI Deep Dive |
| The Women's Pavillion Psc | Health Care and Social Assistance | $291,146 | $292,246 | 34 | $10.7M | AI Deep Dive |
| Acme Plumbing and Heating Company Inc. | Construction | $291,087 | $293,440 | 34 | $10.8M | AI Deep Dive |
| Industrial Mold & Machine Co., Inc. | Manufacturing | $290,500 | $293,214 | 20 | <$5M | AI Deep Dive |
| O'bryan Composting, LLC | Agriculture, Forestry, Fishing and Hunting | $286,693 | $288,178 | 42 | $6.9M | AI Deep Dive |
| Rehabilitation & Performance Institute, Psc | Health Care and Social Assistance | $285,965 | $287,728 | 29 | <$5M | AI Deep Dive |
| Sullivan Mountjoy, Psc | Professional, Scientific, and Technical Services | $283,623 | $285,294 | 18 | <$5M | AI Deep Dive |
| Greenwell Chisholm | Manufacturing | $283,621 | $287,319 | 30 | $29.8M | AI Deep Dive |
| Wbr, Inc. | Construction | $281,087 | $282,904 | 21 | $6.9M | AI Deep Dive |
| Glenn Funeral Home & Crematory, Inc. | Other Services (except Public Administration) | $279,735 | $282,478 | 21 | <$5M | AI Deep Dive |
| Four B Investments LLC | Accommodation and Food Services | $273,014 | $275,327 | 27 | <$5M | AI Deep Dive |
| Ohio Valley Insurance LLC | Finance and Insurance | $269,847 | $272,109 | 23 | $11.8M | AI Deep Dive |
| Summits Obstestrics & Gynecology. Psc | Health Care and Social Assistance | $261,684 | $263,490 | 28 | $8.8M | AI Deep Dive |
| Sam Estes Painting INC | Construction | $260,375 | $262,458 | 34 | $8.6M | AI Deep Dive |
| Wills Animal Hospital, Psc | Professional, Scientific, and Technical Services | $259,142 | $261,556 | 35 | <$5M | AI Deep Dive |
| Owensboro Area Shelter, Information & Services | Other Services (except Public Administration) | $256,975 | $259,570 | 40 | N/A | AI Deep Dive |
| Dahl & Groezinger, INC | Wholesale Trade | $256,500 | $258,673 | 40 | $12.3M | AI Deep Dive |