| Distinctive Home Care INC | Health Care and Social Assistance | $4,306,015 | $4,354,738 | 400 | $68.5M |  Research |
| Rrr Bowie LLC | Retail Trade | $2,854,500 | $2,886,930 | 245 | $61.7M | Research |
| Insight Technology Solutions Inc. | Professional, Scientific, and Technical Services | $2,778,610 | $0 | 175 | $24.5M | Research |
| Immaculate Healthcare Services INC | Health Care and Social Assistance | $2,411,900 | $2,443,188 | 500 | $85.6M | Research |
| International Masonry Training and Education Foundation | Educational Services | $1,571,200 | $1,587,741 | 125 | N/A | Research |
| Ardmore Enterprises INC | Health Care and Social Assistance | $1,492,500 | $1,512,732 | 100 | $17.0M | Research |
| Electrical Training Alliance | Educational Services | $1,420,841 | $1,436,391 | 51 | N/A | Research |
| Ourisman Chevrolet of Bowie INC | Retail Trade | $1,266,100 | $1,028,935 | 99 | $24.9M | Research |
| Darcars of Bowie INC | Retail Trade | $1,072,053 | $1,084,441 | 81 | $20.4M | Research |
| Mitchellville Health Care Ll | Health Care and Social Assistance | $1,008,000 | $1,019,648 | 95 | $16.2M | Research |
| Installnet International, Inc. | Construction | $975,000 | $982,266 | 67 | $22.8M | Research |
| V & V Construction INC | Construction | $953,785 | $961,176 | 64 | $6.9M | Research |
| V & V Construction INC | Construction | $953,782 | $960,864 | 51 | $8.8M | Research |
| O'barr Construction LLC | Construction | $912,700 | $920,502 | 41 | $46.7M | Research |
| St Pius X Church | Other Services (except Public Administration) | $858,600 | $870,120 | 112 | N/A | Research |
| Ashlin Management Group, Inc. | Professional, Scientific, and Technical Services | $857,357 | $866,883 | 36 | <$5M | Research |
| March Hodge Bowie LLC | Retail Trade | $847,262 | $850,883 | 61 | $15.4M | Research |
| Ops Tech Alliance LLC | Professional, Scientific, and Technical Services | $844,600 | $853,972 | 46 | <$5M | Research |
| 3 D Communications LLC | Professional, Scientific, and Technical Services | $833,600 | $839,273 | 41 | <$5M | Research |
| March Hodge Bowie LLC | Retail Trade | $797,857 | $804,939 | 85 | $21.4M | Research |
| Decaro, Doran, Siciliano, Gallagher & Deblasis, LLP | Professional, Scientific, and Technical Services | $785,000 | $790,441 | 52 | $12.6M | Research |
| Trinity Cyber, Inc. | Professional, Scientific, and Technical Services | $781,100 | $785,288 | 38 | $7.9M | Research |
| F & L Construction INC | Administrative and Support and Waste Management | $765,877 | $765,882 | 61 | $11.5M | Research |
| K Cars of Bowie LLC | Retail Trade | $715,200 | $719,829 | 48 | $12.1M | Research |
| Decaro Doran Siciliano Gallagher & Deblasis LLP | Public Administration | $714,000 | $720,358 | 57 | N/A | Research |