| Allied Advertising Limited Partnership | Professional, Scientific, and Technical Services | $5,530,600 | $5,602,422 | 418 | $46.8M |  Research |
| Sumiao Xiang Restaurant LLC | Accommodation and Food Services | $5,025,900 | $372,353 | 28 | <$5M | Research |
| Datalucent Inc. | Information | $4,959,340 | $49,958 | 3 | <$5M | Research |
| Tatte Holdings LLC | Accommodation and Food Services | $4,680,200 | $4,734,183 | 500 | $88.8M | Research |
| Union of Concerned Scientist | Other Services (except Public Administration) | $4,380,848 | $4,418,206 | 231 | $44.1M | Research |
| Baystate Pool Supplies, Inc. | Wholesale Trade | $4,133,700 | $3,896,698 | 284 | $87.0M | Research |
| Cic Innovation Services, LLC | Professional, Scientific, and Technical Services | $3,950,222 | $4,001,575 | 268 | $37.5M | Research |
| Charles River Analytics INC | Professional, Scientific, and Technical Services | $3,782,902 | $1,221,500 | 200 | $41.4M | Research |
| Symmes Maini & Mckee Associates, Inc. | Professional, Scientific, and Technical Services | $3,579,915 | $2,995,316 | 186 | $57.7M | Research |
| John a Penney Co INC | Construction | $3,528,900 | $3,567,283 | 365 | $120.1M | Research |
| Cyclerion Therapeutics, Inc. | Manufacturing | $3,509,355 | $3,564,042 | 88 | $87.3M | Research |
| Aai Associates, Inc. | Professional, Scientific, and Technical Services | $2,384,232 | $2,416,287 | 122 | $37.8M | Research |
| Lycee International De Boston / International School of Bost | Educational Services | $2,200,207 | $2,225,815 | 156 | N/A | Research |
| Myjove Corporation | Information | $2,134,500 | $2,158,418 | 138 | $23.2M | Research |
| Industrial Economics, Incorporated | Professional, Scientific, and Technical Services | $2,116,307 | $0 | 123 | $12.7M | Research |
| Campus Child Care Inc. | Health Care and Social Assistance | $2,114,093 | $1,525,328 | 182 | N/A | Research |
| Sancta Maria Hospital INC | Health Care and Social Assistance | $2,099,400 | $2,123,660 | 149 | N/A | Research |
| Clover Fast Food, Inc. | Accommodation and Food Services | $2,000,000 | $2,022,959 | 188 | $33.4M | Research |
| Commander Properties INC | Accommodation and Food Services | $2,000,000 | $2,015,397 | 105 | $32.8M | Research |
| Forsyth Dental Infirmary for Children | Professional, Scientific, and Technical Services | $1,888,255 | $1,898,705 | 108 | N/A | Research |
| Forsyth Dental Infirmary for Children | Professional, Scientific, and Technical Services | $1,814,900 | $1,824,596 | 108 | N/A | Research |
| Modo Labs, Inc. | Professional, Scientific, and Technical Services | $1,788,824 | $1,804,507 | 88 | $18.2M | Research |
| Forest Properties Management Co INC | Real Estate and Rental and Leasing | $1,746,000 | $1,757,058 | 129 | $19.4M | Research |
| Gns Healthcare INC | Professional, Scientific, and Technical Services | $1,744,855 | $1,765,506 | 73 | $15.2M | Research |
| Michael Van Valkenburgh Associates, Inc. | Professional, Scientific, and Technical Services | $1,686,557 | $1,703,657 | 102 | $20.6M | Research |