| Mark Food Enterprises | Accommodation and Food Services | $10,000,000 | $380,817 | 59 | $10.5M |  Research |
| Nizhoni Health Systems, LLC | Health Care and Social Assistance | $9,407,280 | $9,511,805 | 500 | $85.6M | Research |
| Triumvirate Environmental, Inc. | Administrative and Support and Waste Management | $9,045,899 | $8,706,595 | 500 | $94.6M | Research |
| Formlabs Inc. | Manufacturing | $7,659,820 | $7,762,828 | 484 | $126.7M | Research |
| Cataldo Ambulance Service, Inc. | Health Care and Social Assistance | $5,000,000 | $7,026,641 | 500 | $115.9M | Research |
| Gentle Giant Employment Company LLC | Transportation and Warehousing | $3,500,000 | $3,540,542 | 420 | $104.9M | Research |
| Eastern Bus Company, Inc. | Construction | $2,232,635 | $2,261,287 | 267 | $82.8M | Research |
| Velir Studios INC | Professional, Scientific, and Technical Services | $2,166,107 | $2,190,676 | 111 | $12.4M | Research |
| Eastern Bus Co INC | Construction | $2,000,000 | $2,014,444 | 183 | $56.7M | Research |
| Rentalbeast, Inc. | Real Estate and Rental and Leasing | $2,000,000 | $801,894 | 30 | <$5M | Research |
| Bent Electrical Contractors INC | Construction | $1,943,263 | $1,979,051 | 125 | $41.1M | Research |
| Bent Electrical Contractors, Inc. | Construction | $1,882,300 | $1,902,796 | 70 | $23.0M | Research |
| Candlewick Press INC | Information | $1,860,699 | $1,878,541 | 118 | $19.8M | Research |
| Finch Therapeutics, Inc. | Manufacturing | $1,807,780 | $1,826,762 | 92 | $91.3M | Research |
| Gns Healthcare INC | Professional, Scientific, and Technical Services | $1,794,627 | $1,809,722 | 48 | $10.0M | Research |
| Somerville-Cambridge Elder Services, Inc. | Health Care and Social Assistance | $1,725,950 | $1,738,319 | 147 | N/A | Research |
| Herb Chambers I-93 INC | Retail Trade | $1,608,812 | $1,627,368 | 114 | $28.7M | Research |
| Independent Electric Supply Corp. | Wholesale Trade | $1,488,000 | $1,507,120 | 85 | $26.0M | Research |
| Youthbuild USA INC | Health Care and Social Assistance | $1,470,462 | $1,485,908 | 68 | N/A | Research |
| Galatea Associates, LLC | Professional, Scientific, and Technical Services | $1,438,526 | $1,449,601 | 67 | $13.9M | Research |
| Righthand Robotics, Inc. | Manufacturing | $1,358,578 | $1,371,559 | 66 | $17.3M | Research |
| Abc Moving Services Inc. | Transportation and Warehousing | $1,353,622 | $605,383 | 59 | $14.7M | Research |
| Paint Nite LLC | Arts, Entertainment, and Recreation | $1,337,872 | $1,356,082 | 59 | $15.5M | Research |
| Metropolitan Pipe and Supply Company | Wholesale Trade | $1,297,450 | $1,308,469 | 112 | $34.3M | Research |
| Righthand Robotics Inc. | Manufacturing | $1,293,994 | $0 | 54 | $14.1M | Research |