| American Eagle Lifecare Corporation | Health Care and Social Assistance | $7,554,400 | $7,637,918 | 500 | N/A |  Research |
| Location Services, LLC | Professional, Scientific, and Technical Services | $5,793,261 | $5,850,228 | 488 | $68.3M | Research |
| Mckinley Companies, LLC | Real Estate and Rental and Leasing | $5,626,000 | $5,691,354 | 427 | $64.0M | Research |
| Cayman Chemical Company INC | Manufacturing | $5,341,528 | $5,407,382 | 352 | $349.1M | Research |
| Ervin Industries, Inc. | Manufacturing | $5,092,000 | $5,150,773 | 254 | $251.9M | Research |
| Organ Procurement Agency of Michigan | Health Care and Social Assistance | $4,268,400 | $0 | 235 | N/A | Research |
| Global Educational Excellence, LLC | Educational Services | $4,040,600 | $4,098,852 | 242 | $32.3M | Research |
| Manpower, Inc. of Southeastern Michigan | Administrative and Support and Waste Management | $3,441,060 | $3,481,410 | 415 | $65.3M | Research |
| Varsity Ford | Retail Trade | $3,291,900 | $2,489,881 | 168 | $42.3M | Research |
| Tek Wissen LLC | Professional, Scientific, and Technical Services | $3,108,800 | $3,156,641 | 104 | $10.8M | Research |
| East West Industrial Engineering Co. | Administrative and Support and Waste Management | $3,052,500 | $3,087,519 | 241 | $45.6M | Research |
| Moriah Incorporated | Health Care and Social Assistance | $3,019,100 | $3,044,008 | 474 | $80.6M | Research |
| Gold Star Mortgage Financial Group, Corporation | Finance and Insurance | $2,902,868 | $4,644,320 | 356 | $182.9M | Research |
| Arbor Construction Personnel INC | Construction | $2,599,700 | $2,625,911 | 118 | $27.3M | Research |
| Altarum Institute | Professional, Scientific, and Technical Services | $2,500,000 | $2,528,750 | 235 | N/A | Research |
| Planned Parenthood of Michigan | Health Care and Social Assistance | $2,361,377 | $2,395,277 | 201 | N/A | Research |
| Eversight | Health Care and Social Assistance | $2,246,600 | $2,274,745 | 151 | N/A | Research |
| Regis Construction, LLC | Construction | $2,220,000 | $2,244,755 | 148 | $168.6M | Research |
| Tekwissen LLC | Administrative and Support and Waste Management | $2,000,000 | $2,019,556 | 259 | $40.8M | Research |
| Careevolution | Information | $1,972,600 | $1,993,367 | 87 | $14.6M | Research |
| Hook Studios LLC | Professional, Scientific, and Technical Services | $1,963,700 | $1,985,166 | 117 | $13.1M | Research |
| R.n.a. of Ann Arbor, Incorporated | Administrative and Support and Waste Management | $1,933,857 | $1,951,799 | 354 | $76.7M | Research |
| Arbor Research Collaborative for Health | Professional, Scientific, and Technical Services | $1,862,400 | $1,723,746 | 106 | N/A | Research |
| B/d Ops, LLC | Management of Companies and Enterprises | $1,817,400 | $1,831,292 | 124 | $20.4M | Research |
| Zingerman's Delicatessen Inc. | Accommodation and Food Services | $1,815,986 | $1,825,190 | 129 | $22.9M | Research |