| Byers Wrecker Service Inc. | Transportation and Warehousing | $321,754 | $323,595 | 25 | <$5M | AI Deep Dive |
| Paletz Law Firm PC | Professional, Scientific, and Technical Services | $318,952 | $320,551 | 26 | $6.3M | AI Deep Dive |
| Irish Beer Works Rochester INC | Accommodation and Food Services | $315,701 | $319,005 | 35 | $6.2M | AI Deep Dive |
| Parkbridge Investment Group INC | Real Estate and Rental and Leasing | $314,900 | $317,480 | 60 | $7.0M | AI Deep Dive |
| Results in Education Foundation | Other Services (except Public Administration) | $313,937 | $316,190 | 16 | N/A | AI Deep Dive |
| Tom Furnari Painting, LLC | Construction | $310,149 | $313,290 | 37 | $9.3M | AI Deep Dive |
| Auxiom, LLC | Professional, Scientific, and Technical Services | $308,600 | $310,569 | 20 | <$5M | AI Deep Dive |
| Neuron Publishing LLC | Manufacturing | $308,487 | $312,308 | 34 | $33.7M | AI Deep Dive |
| Surge Solutions, LLC | Professional, Scientific, and Technical Services | $307,800 | $289,457 | 16 | <$5M | AI Deep Dive |
| Fourthstreet LLC | Accommodation and Food Services | $290,118 | $291,919 | 50 | $8.9M | AI Deep Dive |
| Results in Education Foundation | Other Services (except Public Administration) | $286,500 | $289,742 | 15 | N/A | AI Deep Dive |
| Tour Connection Incorporated | Professional, Scientific, and Technical Services | $286,417 | $288,062 | 14 | <$5M | AI Deep Dive |
| The Whims Insurance Agency INC | Finance and Insurance | $285,600 | $199,358 | 18 | $9.2M | AI Deep Dive |
| Goodison Veterinary Center, P.c. | Professional, Scientific, and Technical Services | $282,800 | $285,527 | 33 | <$5M | AI Deep Dive |
| Rochester General Surgery Plc | Health Care and Social Assistance | $277,900 | $281,258 | 22 | $6.9M | AI Deep Dive |
| Rochester Dermatology Clinic, P.c. | Health Care and Social Assistance | $276,967 | $279,698 | 19 | $6.0M | AI Deep Dive |
| Tour Connection Incorporated | Professional, Scientific, and Technical Services | $276,422 | $278,491 | 14 | <$5M | AI Deep Dive |
| Resourcestream Inc. | Information | $268,915 | $271,955 | 13 | <$5M | AI Deep Dive |
| Doolin & Haddad P.l.c. | Health Care and Social Assistance | $253,902 | $255,568 | 12 | <$5M | AI Deep Dive |
| Doolin & Haddad, P.l.c. | Health Care and Social Assistance | $253,890 | $255,991 | 13 | <$5M | AI Deep Dive |
| Bxb Media, LLC | Professional, Scientific, and Technical Services | $249,813 | $251,284 | 31 | <$5M | AI Deep Dive |
| Efthemios Raphtis, M.d., P.c. DBA Balian Eye Center | Health Care and Social Assistance | $247,885 | $249,680 | 27 | $6.3M | AI Deep Dive |
| French Associates INC | Professional, Scientific, and Technical Services | $246,900 | $249,156 | 13 | <$5M | AI Deep Dive |
| Premier Surgical Specialists, PC | Health Care and Social Assistance | $245,597 | $247,804 | 30 | $9.4M | AI Deep Dive |
| Walby Dental Pllc | Health Care and Social Assistance | $242,700 | $244,406 | 23 | $5.3M | AI Deep Dive |