| J & B Pallet, LLC | Manufacturing | $1,027,700 | $1,036,521 | 78 | $77.4M |  Research |
| Valley Craft Industries Inc. | Manufacturing | $913,700 | $920,604 | 83 | $21.7M | Research |
| Valley Craft Industries INC | Manufacturing | $882,095 | $886,456 | 79 | $19.3M | Research |
| Pepin Manufacturing, INC | Manufacturing | $585,600 | $589,715 | 74 | $19.4M | Research |
| J&b Lakeland Logistics | Manufacturing | $394,700 | $398,088 | 67 | $66.5M | Research |
| Kohlnhofer Farms INC | Agriculture, Forestry, Fishing and Hunting | $394,400 | $379,783 | 39 | $6.4M | Research |
| Acrotech, Inc. | Manufacturing | $393,700 | $396,609 | 30 | $29.8M | Research |
| Kohlnhofer Farms | Agriculture, Forestry, Fishing and Hunting | $374,997 | $378,570 | 27 | <$5M | Research |
| Scotch Prairie Farms LLC | Agriculture, Forestry, Fishing and Hunting | $265,999 | $268,504 | 35 | $5.7M | Research |
| Jmc Computer Service, Inc. | Professional, Scientific, and Technical Services | $225,600 | $227,041 | 17 | <$5M | Research |
| Bluff Country School Bus Service, Inc. | Transportation and Warehousing | $211,200 | $212,731 | 65 | $25.0M | Research |
| Hagedorn Enterprises, Inc. | Administrative and Support and Waste Management | $203,300 | $204,701 | 25 | <$5M | Research |
| Railhouse Holdings INC | Accommodation and Food Services | $195,659 | $196,506 | 25 | <$5M | Research |
| Lakeland Transfer LLC | Transportation and Warehousing | $185,400 | $186,986 | 19 | $5.1M | Research |
| River Oaks at Lake Pepin | Health Care and Social Assistance | $184,932 | $154,195 | 30 | $5.1M | Research |
| Automation Services, Inc. | Wholesale Trade | $184,500 | $185,761 | 12 | <$5M | Research |
| Enterprise North Inc. | Health Care and Social Assistance | $183,205 | $184,065 | 22 | N/A | Research |
| Engineering Laboratory Design Co., Inc. | Manufacturing | $174,900 | $176,139 | 10 | <$5M | Research |
| Anderson House Tours, Inc. | Transportation and Warehousing | $173,700 | $175,809 | 20 | $7.7M | Research |
| Anderson House Tours, Inc. | Administrative and Support and Waste Management | $173,700 | $175,601 | 20 | $6.3M | Research |
| Engineering Laboratory Design Co. Inc. | Manufacturing | $165,372 | $166,365 | 10 | <$5M | Research |
| Pepin Valley Dental P.a. | Health Care and Social Assistance | $158,787 | $159,613 | 15 | <$5M | Research |
| Otago, Inc. | Accommodation and Food Services | $158,700 | $159,718 | 56 | $9.9M | Research |
| Pepin Valley Dental, P.a. | Health Care and Social Assistance | $150,800 | $151,914 | 15 | <$5M | Research |
| The Jewel, LLC | Arts, Entertainment, and Recreation | $150,500 | $151,637 | 58 | $8.1M | Research |