| Valley Computer Solutions, LLC | Professional, Scientific, and Technical Services | $76,485 | $76,961 | 7 | <$5M | AI Deep Dive |
| Rohrer Center Ltd | Health Care and Social Assistance | $76,300 | $74,187 | 9 | <$5M | AI Deep Dive |
| Ddi of Winona DBA Taco Johns | Accommodation and Food Services | $76,263 | $76,925 | 22 | <$5M | AI Deep Dive |
| Craig Woodworth | Real Estate and Rental and Leasing | $75,900 | $76,450 | 8 | <$5M | AI Deep Dive |
| Wabasha Ready Mix L. L. C. | Manufacturing | $75,497 | $76,063 | 9 | $8.9M | AI Deep Dive |
| Mike Sherman Plumbing and Heating INC | Construction | $75,000 | $75,556 | 7 | <$5M | AI Deep Dive |
| Minnesota City Custom Wear Inc. | Retail Trade | $73,000 | $73,345 | 12 | <$5M | AI Deep Dive |
| Los Mangos Inc. | Accommodation and Food Services | $72,500 | $73,024 | 14 | <$5M | AI Deep Dive |
| Auto Collision Specialists, Inc. | Other Services (except Public Administration) | $72,292 | $72,794 | 8 | <$5M | AI Deep Dive |
| Ocean Sushi INC | Accommodation and Food Services | $72,171 | $72,809 | 20 | <$5M | AI Deep Dive |
| Cfs Contractors, Inc. | Construction | $72,100 | $72,633 | 7 | <$5M | AI Deep Dive |
| Sim Sound & Video INC | Construction | $71,610 | $71,956 | 7 | <$5M | AI Deep Dive |
| Leo Smith IV | Accommodation and Food Services | $71,165 | $71,556 | 9 | <$5M | AI Deep Dive |
| Rohrer Center Ltd | Health Care and Social Assistance | $71,105 | $71,478 | 7 | <$5M | AI Deep Dive |
| Robeson Family Vision Center, Inc. | Health Care and Social Assistance | $70,700 | $71,291 | 8 | <$5M | AI Deep Dive |
| Winona Area Chamber of Commerce | Other Services (except Public Administration) | $70,485 | $70,859 | 5 | <$5M | AI Deep Dive |
| Westgate Lanes INC | Arts, Entertainment, and Recreation | $70,440 | $70,712 | 18 | <$5M | AI Deep Dive |
| Sport & Spine Physical Therapy of Winona, Inc. | Health Care and Social Assistance | $70,300 | $70,630 | 7 | <$5M | AI Deep Dive |
| Boomer's Quality Plumbing LLC | Construction | $69,300 | $69,807 | 6 | <$5M | AI Deep Dive |
| Blooming Grounds LLC | Accommodation and Food Services | $68,900 | $69,374 | 11 | <$5M | AI Deep Dive |
| Holtans Jewelry INC | Retail Trade | $68,500 | $69,086 | 6 | <$5M | AI Deep Dive |
| Management Recruiters of Winona Inc. | Professional, Scientific, and Technical Services | $67,500 | $68,008 | 6 | <$5M | AI Deep Dive |
| Kickass Snacks INC | Retail Trade | $67,100 | $67,598 | 5 | <$5M | AI Deep Dive |
| Immaculate Heart of Mary Seminary | Other Services (except Public Administration) | $67,000 | $67,456 | 6 | <$5M | AI Deep Dive |
| Bettermilk INC | Agriculture, Forestry, Fishing and Hunting | $66,500 | $66,879 | 5 | <$5M | AI Deep Dive |