| Mississippi Ag Company | Wholesale Trade | $3,658,000 | $3,700,575 | 288 | $88.2M |  Research |
| First Choice Home Care, LLC | Health Care and Social Assistance | $1,754,891 | $1,770,783 | 500 | $81.0M | Research |
| Shackelford Construction & Hauling LLC | Construction | $1,358,300 | $1,373,053 | 90 | $27.9M | Research |
| Simmons Farm Raised Catfish INC | Manufacturing | $1,257,658 | $1,265,797 | 199 | $70.2M | Research |
| Simmons Farm Raised Catfish, INC | Manufacturing | $1,257,600 | $1,265,809 | 209 | $73.8M | Research |
| Shackelford Construction & Hauling LLC | Construction | $1,086,000 | $1,095,834 | 84 | $26.0M | Research |
| Yazoo Valley Electric Power Association INC | Utilities | $855,731 | $860,556 | 55 | N/A | Research |
| Nexion Health at Yazoo City, Inc. | Health Care and Social Assistance | $408,746 | $413,231 | 173 | $29.4M | Research |
| Yazoo Lumber & Mats LLC | Manufacturing | $313,259 | $315,208 | 16 | $15.9M | Research |
| Yazoo Lumber & Mats, LLC | Wholesale Trade | $313,200 | $315,010 | 36 | $11.0M | Research |
| Phillips Brothers Farms LLC | Agriculture, Forestry, Fishing and Hunting | $292,970 | $293,857 | 39 | $6.4M | Research |
| J & J Bagging, LLC | Wholesale Trade | $270,378 | $272,052 | 82 | $21.1M | Research |
| Manchester Educational Foundation, Inc. | Educational Services | $268,711 | $270,249 | 49 | N/A | Research |
| Manchester Educational Foundation | Educational Services | $268,711 | $270,271 | 30 | N/A | Research |
| Eunice Linburger | Retail Trade | $220,800 | $0 | 12 | <$5M | Research |
| Action Management LLC | Real Estate and Rental and Leasing | $190,028 | $191,897 | 13 | <$5M | Research |
| Action Management LLC | Real Estate and Rental and Leasing | $190,027 | $192,328 | 13 | <$5M | Research |
| Crabtree Manufacturing INC | Manufacturing | $184,235 | $185,146 | 20 | $5.2M | Research |
| Yazoo Family Medicine PA | Health Care and Social Assistance | $171,680 | $173,416 | 17 | $5.3M | Research |
| Yazoo Family Medicine PA | Health Care and Social Assistance | $171,600 | $173,392 | 13 | <$5M | Research |
| Phillips Brothers Farms, LLC | Agriculture, Forestry, Fishing and Hunting | $145,600 | $258,191 | 18 | <$5M | Research |
| Aa Calibration Services, LLC | Professional, Scientific, and Technical Services | $137,500 | $139,063 | 12 | <$5M | Research |
| Dutch Brake Fisheries LLC | Agriculture, Forestry, Fishing and Hunting | $134,536 | $135,474 | 15 | <$5M | Research |
| Boys & Girls Club of the Mississippi Delta | Health Care and Social Assistance | $132,000 | $132,987 | 13 | N/A | Research |
| Dutch Brake Fisheries LLC | Agriculture, Forestry, Fishing and Hunting | $122,900 | $123,637 | 16 | <$5M | Research |