| Bolivar Farmers Exchange | Wholesale Trade | $404,100 | $406,514 | 60 | $15.4M |  Research |
| Quarles Supply Co., INC | Wholesale Trade | $396,300 | $399,030 | 48 | $14.7M | Research |
| Bill Roberts Chevrolet-Pontiac-Olds-Buic | Retail Trade | $385,400 | $387,184 | 38 | $9.6M | Research |
| Bill Roberts Chevrolet Buick, Inc. | Retail Trade | $385,400 | $388,747 | 36 | $9.1M | Research |
| Home Court Advantage Inc. | Health Care and Social Assistance | $358,700 | $360,872 | 65 | $10.5M | Research |
| Douglas, Haun & Heidemann, PC | Professional, Scientific, and Technical Services | $358,100 | $360,627 | 30 | $7.3M | Research |
| Douglas Haun & Heidemann P.c. | Professional, Scientific, and Technical Services | $354,158 | $356,116 | 28 | $6.8M | Research |
| Texas County Technical Institute INC | Educational Services | $313,100 | $314,910 | 40 | N/A | Research |
| Smith's Restaurant LLC | Accommodation and Food Services | $303,529 | $305,350 | 72 | $12.8M | Research |
| Bill Grant Ford Inc. | Retail Trade | $289,274 | $291,050 | 25 | $6.3M | Research |
| Pomeroy Services, LLC | Construction | $283,290 | $285,863 | 25 | $7.7M | Research |
| Hobbytime Motorsports, INC | Retail Trade | $236,200 | $238,700 | 21 | $5.5M | Research |
| Creator Designs, INC | Manufacturing | $234,600 | $235,897 | 35 | $34.7M | Research |
| Affordable Towing Servicing Southwest Missouri, L.l.c. | Transportation and Warehousing | $232,100 | $233,273 | 47 | <$5M | Research |
| Wommack Mounment Co. | Retail Trade | $218,700 | $220,055 | 17 | <$5M | Research |
| Smiths Restaurant LLC | Accommodation and Food Services | $212,400 | $213,952 | 70 | $12.4M | Research |
| Mcdonald's of Bolivar, INC | Accommodation and Food Services | $210,200 | $211,536 | 57 | $10.1M | Research |
| Endrizzi Diesel LLC | Other Services (except Public Administration) | $187,700 | $161,310 | 13 | <$5M | Research |
| A-1 Tool, INC | Manufacturing | $187,100 | $188,165 | 19 | <$5M | Research |
| Polk County Health Center | Health Care and Social Assistance | $185,400 | $187,290 | 27 | N/A | Research |
| Capstone Insurors INC | Finance and Insurance | $185,000 | $213,479 | 21 | $10.8M | Research |
| Farmers Mkt of Bolivar Mo INC | Retail Trade | $165,377 | $166,310 | 16 | <$5M | Research |
| Jump Dental LLC | Health Care and Social Assistance | $146,519 | $147,252 | 10 | <$5M | Research |
| Classic Beauty Supply Co | Retail Trade | $145,300 | $146,273 | 28 | <$5M | Research |
| Fabricated Extrusion Company of Missouri LLC | Manufacturing | $142,900 | $143,750 | 17 | $16.9M | Research |