| Phillips Hardy, INC | Construction | $1,940,300 | $1,962,128 | 73 | $22.6M |  Research |
| Sisters Supportive Living, INC | Health Care and Social Assistance | $648,360 | $653,457 | 102 | $17.4M | Research |
| Unlimited Opportunities, INC | Health Care and Social Assistance | $580,189 | $583,289 | 110 | N/A | Research |
| Greis Trucking & Excavating INC | Construction | $515,316 | $518,140 | 42 | $13.0M | Research |
| Potter Transport, Inc. | Transportation and Warehousing | $437,000 | $439,933 | 32 | $8.5M | Research |
| Potter Transport, Inc. | Transportation and Warehousing | $432,035 | $434,343 | 24 | $5.5M | Research |
| Gerding, Korte, and Chitwood, Pc, Cpa's | Professional, Scientific, and Technical Services | $339,553 | $341,788 | 22 | $7.0M | Research |
| Cooper Sales & Welding LLC | Other Services (except Public Administration) | $329,012 | $330,977 | 39 | $8.4M | Research |
| Rick Ball Chevrolet Buick Gmc Cadillac, Inc. | Retail Trade | $268,200 | $270,045 | 25 | $6.3M | Research |
| Central Missouri Glass & More LLC | Construction | $265,792 | $267,314 | 27 | $6.2M | Research |
| Central Missouri Glass & More, LLC | Construction | $253,841 | $255,232 | 22 | $5.1M | Research |
| W-K Ford, Inc. | Retail Trade | $252,131 | $254,099 | 21 | $5.3M | Research |
| Ashley Manor, Inc. | Health Care and Social Assistance | $237,900 | $239,704 | 33 | $5.6M | Research |
| Ss. Peter and Paul Catholic Church, Boonville | Other Services (except Public Administration) | $215,200 | $216,425 | 39 | N/A | Research |
| A & Km, Inc. | Accommodation and Food Services | $168,700 | $170,521 | 45 | $8.0M | Research |
| Boonslick Heartland Young Men's Christian Association | Other Services (except Public Administration) | $157,292 | $158,083 | 66 | N/A | Research |
| Boonslick Heartland Young Men's Christian Association | Health Care and Social Assistance | $156,968 | $157,853 | 62 | N/A | Research |
| Lexcorp of Columbia, Inc. | Accommodation and Food Services | $147,700 | $149,141 | 40 | $7.1M | Research |
| Miller Medical Arts Pharmacy, Inc. | Retail Trade | $147,700 | $148,537 | 18 | <$5M | Research |
| Frontier Property Mgmt., LLC | Real Estate and Rental and Leasing | $107,400 | $108,445 | 14 | <$5M | Research |
| K-Rose, Inc. | Accommodation and Food Services | $101,700 | $102,698 | 40 | $7.1M | Research |
| W-K Automotive, Inc. | Retail Trade | $96,505 | $97,258 | 11 | <$5M | Research |
| Cooper County Animal Hospital PC | Professional, Scientific, and Technical Services | $94,143 | $94,804 | 11 | <$5M | Research |
| Hoff Farms INC | Agriculture, Forestry, Fishing and Hunting | $87,800 | $88,551 | 5 | <$5M | Research |
| Bradley Automotive LLC | Retail Trade | $81,186 | $81,797 | 14 | <$5M | Research |