| Central Methodist University | Educational Services | $3,825,000 | $3,870,050 | 424 | N/A |  Research |
| Complete Health Care Solutions, INC | Health Care and Social Assistance | $1,247,725 | $1,255,416 | 225 | $38.3M | Research |
| Missouri-Pacific Lumber Co INC | Manufacturing | $425,800 | $430,291 | 41 | $40.7M | Research |
| Missouri Pacific Lumber Company, INC | Wholesale Trade | $366,700 | $401,402 | 37 | $11.3M | Research |
| Addison Biological Laboratory, INC | Professional, Scientific, and Technical Services | $337,087 | $338,896 | 27 | $5.6M | Research |
| Sunderland Trucking LLC | Transportation and Warehousing | $305,097 | $307,461 | 87 | $17.5M | Research |
| Howard County Home Health and Hospice | Health Care and Social Assistance | $273,590 | $275,133 | 25 | N/A | Research |
| Sunderland Trucking LLC | Transportation and Warehousing | $229,908 | $231,338 | 18 | <$5M | Research |
| Endless Options, INC | Health Care and Social Assistance | $218,880 | $221,009 | 54 | $9.2M | Research |
| N and R of Fayette INC | Health Care and Social Assistance | $157,900 | $159,721 | 42 | $7.1M | Research |
| Cook Concrete Construction Company | Construction | $152,200 | $153,409 | 12 | <$5M | Research |
| Endeavor Agency Inc. | Administrative and Support and Waste Management | $138,098 | $138,826 | 13 | <$5M | Research |
| Endeavor Agency | Administrative and Support and Waste Management | $132,990 | $133,829 | 14 | <$5M | Research |
| Linda Perkins Home, INC | Health Care and Social Assistance | $102,199 | $102,759 | 22 | <$5M | Research |
| Hart's Diesel INC | Manufacturing | $95,000 | $95,480 | 13 | <$5M | Research |
| Harts Diesel INC | Manufacturing | $95,000 | $95,488 | 13 | <$5M | Research |
| Hank Ventures, INC | Health Care and Social Assistance | $93,895 | $94,430 | 27 | <$5M | Research |
| Hilgedick Electric Service, INC | Construction | $84,767 | $85,172 | 10 | <$5M | Research |
| Inovatia Laboratories LLC | Health Care and Social Assistance | $80,621 | $81,105 | 8 | <$5M | Research |
| Emmet's Kitchen and Tap | Accommodation and Food Services | $76,000 | $76,408 | 26 | <$5M | Research |
| Earl Veterinary Supply, Inc. | Retail Trade | $68,696 | $69,106 | 10 | <$5M | Research |
| Howard County Veterinary Services, PC | Professional, Scientific, and Technical Services | $64,938 | $65,596 | 9 | <$5M | Research |
| Fayette Dos Primos, LLC | Accommodation and Food Services | $46,700 | $47,251 | 7 | <$5M | Research |
| Jessica L Quint, DDS | Health Care and Social Assistance | $42,597 | $42,808 | 6 | <$5M | Research |
| Donald and Terry Sanders | Agriculture, Forestry, Fishing and Hunting | $41,667 | $41,927 | 22 | <$5M | Research |