| Bleigh Construction Company | Construction | $1,829,420 | $1,843,903 | 161 | $183.4M |  Research |
| Northeast Missouri Electric Power Cooperative | Utilities | $1,507,000 | $1,523,954 | 60 | N/A | Research |
| Doyle Equipment Manufacturing Co | Manufacturing | $1,427,697 | $1,438,841 | 135 | $35.3M | Research |
| Leeser Tx, INC | Transportation and Warehousing | $759,900 | $767,752 | 50 | $10.1M | Research |
| Missouri Rural Electric Cooperative | Other Services (except Public Administration) | $578,919 | $582,602 | 25 | <$5M | Research |
| Port Industries INC | Manufacturing | $526,425 | $529,686 | 46 | $12.0M | Research |
| Riverview Manufacturing INC | Manufacturing | $405,270 | $408,884 | 48 | $12.6M | Research |
| Pro Med LLC | Wholesale Trade | $204,447 | $206,355 | 15 | <$5M | Research |
| Farmers Cooperative Services | Other Services (except Public Administration) | $198,118 | $199,316 | 22 | <$5M | Research |
| Mark Twain Holding Company, LLC | Manufacturing | $191,782 | $193,828 | 35 | $34.7M | Research |
| Klocke INC | Construction | $191,498 | $192,748 | 34 | $10.5M | Research |
| Farmers Cooperative Service | Public Administration | $167,144 | $168,550 | 20 | N/A | Research |
| Bud's Paint & Body Shop, Inc. | Other Services (except Public Administration) | $146,457 | $147,742 | 11 | <$5M | Research |
| Burch Grain Farms | Agriculture, Forestry, Fishing and Hunting | $142,095 | $142,522 | 16 | <$5M | Research |
| Ln Bross Co. Inc. | Manufacturing | $135,505 | $136,295 | 17 | <$5M | Research |
| Clearbridge Display, LLC | Manufacturing | $120,332 | $121,298 | 9 | <$5M | Research |
| Clearbridge Display LLC | Manufacturing | $120,254 | $120,942 | 15 | <$5M | Research |
| Burch Grain Farms | Agriculture, Forestry, Fishing and Hunting | $118,555 | $119,582 | 16 | <$5M | Research |
| Brown's Furniture, Inc. | Retail Trade | $116,868 | $118,056 | 25 | <$5M | Research |
| Tlc Sutter Home, LLC | Health Care and Social Assistance | $97,500 | $97,941 | 28 | <$5M | Research |
| Northeast Missouri Machine & Pattern Co. | Manufacturing | $90,592 | $91,440 | 10 | <$5M | Research |
| Northeast Missouri Machine and Pattern Co | Manufacturing | $90,071 | $90,499 | 8 | <$5M | Research |
| Maher Brothers INC | Agriculture, Forestry, Fishing and Hunting | $86,702 | $87,066 | 11 | <$5M | Research |
| Kb Therapy Inc. | Health Care and Social Assistance | $85,968 | $86,396 | 10 | <$5M | Research |
| Janes Surveying, Inc. | Professional, Scientific, and Technical Services | $85,620 | $86,336 | 12 | <$5M | Research |