| Washington County Memorial Hospital | Health Care and Social Assistance | $2,235,335 | $2,259,771 | 202 | $40.1M |  Research |
| Great Mines Health Center | Administrative and Support and Waste Management | $778,917 | $786,173 | 69 | N/A | Research |
| Reed Lumber Co., LLC | Wholesale Trade | $438,200 | $441,839 | 70 | $21.4M | Research |
| Georgian Gardens Opco LLC | Health Care and Social Assistance | $387,175 | $389,784 | 90 | $15.3M | Research |
| Politte Ready Mix, LLC | Manufacturing | $356,000 | $358,294 | 33 | $32.7M | Research |
| Washington County Handicapped Services Inc. | Health Care and Social Assistance | $266,820 | $268,428 | 45 | N/A | Research |
| Washington County Handicapped Services INC | Health Care and Social Assistance | $266,800 | $269,154 | 45 | N/A | Research |
| Potosi Manor, INC | Health Care and Social Assistance | $247,200 | $250,017 | 59 | $10.0M | Research |
| Washington County Aggregates, Inc. | Mining, Quarrying, and Oil and Gas Extraction | $207,615 | $209,282 | 23 | <$5M | Research |
| Juliette Properties & Investments INC | Retail Trade | $174,744 | $175,783 | 12 | <$5M | Research |
| Extreme Pro Services LLC | Mining, Quarrying, and Oil and Gas Extraction | $134,877 | $136,122 | 16 | <$5M | Research |
| Austin Plaza Pharamacy, INC | Retail Trade | $115,957 | $116,910 | 13 | <$5M | Research |
| Pharmax INC | Health Care and Social Assistance | $111,100 | $111,893 | 14 | <$5M | Research |
| El Ranchito Corporation | Accommodation and Food Services | $91,200 | $92,099 | 14 | <$5M | Research |
| Community Care Pharmacy LLC | Retail Trade | $82,000 | $82,672 | 10 | <$5M | Research |
| Declue Family Funeral Home, LLC | Other Services (except Public Administration) | $77,200 | $77,951 | 11 | <$5M | Research |
| Juliette & Hubbard Investments Inc. | Accommodation and Food Services | $65,859 | $66,312 | 27 | <$5M | Research |
| Robart Rehabilitation & Wellness LLC | Health Care and Social Assistance | $51,400 | $51,660 | 8 | <$5M | Research |
| Big Papas Dough Company, LLC | Accommodation and Food Services | $48,854 | $48,993 | 10 | <$5M | Research |
| Bbmb Inc. | Wholesale Trade | $47,617 | $47,944 | 9 | <$5M | Research |
| The Independant Journal | Information | $46,850 | $47,109 | 10 | <$5M | Research |
| Dna Drug Testing Solutions LLC | Health Care and Social Assistance | $46,772 | $47,060 | 7 | <$5M | Research |
| Dna Drug Testing Solutions LLC | Professional, Scientific, and Technical Services | $46,772 | $46,996 | 7 | <$5M | Research |
| Boyer Lumber Co., Inc. | Wholesale Trade | $44,568 | $44,816 | 10 | <$5M | Research |
| S&h Underground LLC | Construction | $43,169 | $43,409 | 6 | <$5M | Research |