| Erie County Medical Center Corporation | Health Care and Social Assistance | $10,000,000 | $10,109,863 | 500 | N/A |  Research |
| Buffalo Medical Group, P.c. | Health Care and Social Assistance | $10,000,000 | $10,134,521 | 500 | $156.9M | Research |
| Kelly Cooling and Heating | Construction | $10,000,000 | $18,438 | 1 | <$5M | Research |
| Ferguson Electric Construction Co., Inc. | Construction | $10,000,000 | $10,115,068 | 450 | $148.1M | Research |
| New Era Cap Co.,inc. | Wholesale Trade | $8,386,131 | $8,482,572 | 488 | $125.6M | Research |
| Hodgson Russ LLP | Professional, Scientific, and Technical Services | $6,451,700 | $5,936,438 | 364 | $88.5M | Research |
| Freed Maxick Cpas, P.c. | Professional, Scientific, and Technical Services | $5,424,742 | $5,486,569 | 307 | $97.7M | Research |
| Skyworks, L.l.c. | Real Estate and Rental and Leasing | $5,387,930 | $5,448,895 | 360 | $162.7M | Research |
| Ehs, Inc. | Health Care and Social Assistance | $5,169,100 | $5,235,378 | 423 | N/A | Research |
| Rosina Food Products, Inc. | Manufacturing | $4,829,770 | $4,829,808 | 449 | $158.5M | Research |
| Mollenberg-Betz INC | Construction | $4,617,500 | $4,671,012 | 173 | $54.7M | Research |
| Capital Management Services, L.p. | Finance and Insurance | $4,531,100 | $0 | 361 | $185.5M | Research |
| Phillips Lytle LLP | Professional, Scientific, and Technical Services | $4,506,235 | $6,053,949 | 271 | $65.9M | Research |
| Imagine Staffing Technology INC | Administrative and Support and Waste Management | $4,364,065 | $4,439,749 | 379 | $59.6M | Research |
| Academic Medicine Services, Inc. | Health Care and Social Assistance | $4,313,147 | $4,362,660 | 240 | N/A | Research |
| 10 Ellicott Square Court Corporation | Real Estate and Rental and Leasing | $4,285,902 | $4,333,458 | 499 | $74.8M | Research |
| University Emergency Medical Services, INC | Health Care and Social Assistance | $4,231,995 | $4,279,996 | 190 | $30.8M | Research |
| Dunn Tire LLC | Retail Trade | $4,209,074 | $4,268,693 | 450 | $121.7M | Research |
| Sonwil Distribution Center, Inc. | Transportation and Warehousing | $4,157,905 | $4,211,787 | 448 | $113.7M | Research |
| Excelsior Orthopaedics, LLP | Health Care and Social Assistance | $4,065,772 | $4,123,695 | 401 | $125.9M | Research |
| Windsong Radiology Group, PC | Health Care and Social Assistance | $3,948,197 | $3,992,113 | 309 | $71.6M | Research |
| Dival Safety Equipment, Inc. | Wholesale Trade | $3,869,500 | $3,912,330 | 318 | $97.4M | Research |
| Harmac Medical Products | Manufacturing | $3,850,300 | $3,900,512 | 397 | $110.9M | Research |
| Gateway Longview, Inc. | Health Care and Social Assistance | $3,835,472 | $3,884,965 | 353 | N/A | Research |
| Catholic Charities of Buffalo, NY | Other Services (except Public Administration) | $3,743,964 | $3,791,661 | 410 | $78.3M | Research |