| Cornell Cooperative Extension Association Tompkins County | Educational Services | $789,900 | $799,116 | 210 | N/A |  Research |
| Family and Children's Service of Ithaca, INC | Health Care and Social Assistance | $775,500 | $675,643 | 65 | N/A | Research |
| Family Medicine Associates of Ithaca | Health Care and Social Assistance | $741,179 | $747,595 | 41 | $12.9M | Research |
| Sciarabba Walker & Co., LLP | Professional, Scientific, and Technical Services | $740,750 | $744,667 | 53 | $16.9M | Research |
| 360 Campaign Consulting LLC | Professional, Scientific, and Technical Services | $735,310 | $741,515 | 152 | $15.7M | Research |
| Family Medicine Assoc. of Ithaca LLP | Health Care and Social Assistance | $730,700 | $739,228 | 41 | $12.9M | Research |
| Gimme Coffee Inc. | Accommodation and Food Services | $726,459 | $732,191 | 41 | $7.3M | Research |
| Ursa Space Systems Inc. | Educational Services | $724,500 | $732,047 | 42 | $5.5M | Research |
| Holt Architects P.c. | Professional, Scientific, and Technical Services | $716,158 | $704,197 | 36 | $11.2M | Research |
| Holt Architects, PC | Professional, Scientific, and Technical Services | $716,000 | $720,355 | 42 | $13.0M | Research |
| Visiting Nurse Service of Ithaca and Tompkins County | Health Care and Social Assistance | $710,777 | $705,055 | 92 | N/A | Research |
| Hospicare and Palliative Care Services of Tompkins County, I | Health Care and Social Assistance | $680,750 | $689,255 | 71 | N/A | Research |
| Bangs Ambulance, INC | Health Care and Social Assistance | $673,300 | $678,537 | 89 | $20.6M | Research |
| Bangs Ambulance | Health Care and Social Assistance | $673,300 | $677,882 | 89 | $20.6M | Research |
| Renovus Energy INC | Construction | $670,200 | $675,524 | 57 | $18.8M | Research |
| Ithaca Alpha House Center Inc. | Health Care and Social Assistance | $652,247 | $658,037 | 71 | N/A | Research |
| Incodema3d LLC | Manufacturing | $651,888 | $655,139 | 46 | $11.2M | Research |
| Maguire Ford, Inc. | Retail Trade | $624,250 | $627,705 | 59 | $14.9M | Research |
| Asthma & Allergy Associates P.c. | Health Care and Social Assistance | $616,515 | $623,846 | 60 | $18.8M | Research |
| Classen Home Health Associates, Inc. | Health Care and Social Assistance | $593,000 | $596,655 | 132 | $22.6M | Research |
| Tompkins County Public Library | Information | $589,865 | $593,356 | 43 | N/A | Research |
| Bridges Senior Concepts, Inc. | Health Care and Social Assistance | $586,250 | $591,727 | 64 | $10.9M | Research |
| Miller Mayer LLP | Professional, Scientific, and Technical Services | $584,855 | $590,079 | 23 | $5.6M | Research |
| Taughannock Aviation Corp | Transportation and Warehousing | $579,298 | $581,408 | 35 | $8.9M | Research |
| Northeast Pediatrics and Adolescent Medicine | Health Care and Social Assistance | $575,933 | $579,420 | 46 | $14.4M | Research |