| Signature Nissan INC | Retail Trade | $325,355 | $328,724 | 29 | $7.3M | AI Deep Dive |
| Moon Brook Country Club Inc. | Arts, Entertainment, and Recreation | $324,193 | $326,484 | 74 | N/A | AI Deep Dive |
| Corvus Bus & Charter, Inc. | Transportation and Warehousing | $320,720 | $323,154 | 78 | $30.0M | AI Deep Dive |
| Jamestown Electro Plating Works Inc. | Manufacturing | $304,652 | $307,924 | 36 | $8.8M | AI Deep Dive |
| Jamestown Mattress Company Inc. | Manufacturing | $304,500 | $306,818 | 31 | $8.1M | AI Deep Dive |
| Jamestown Mattress Company, Inc. | Manufacturing | $304,500 | $306,936 | 31 | $8.1M | AI Deep Dive |
| Young Women's Christian Association of Jamestown N.y. - Owne | Health Care and Social Assistance | $301,790 | $304,494 | 28 | <$5M | AI Deep Dive |
| Chautauqua Pt, Ot & Slp Professionals, Pllc | Health Care and Social Assistance | $300,142 | $302,979 | 29 | $7.8M | AI Deep Dive |
| Young Women's Christian Association of Jamestown New York | Health Care and Social Assistance | $299,197 | $302,099 | 53 | $8.6M | AI Deep Dive |
| Naco Truck Leasing, Inc. | Transportation and Warehousing | $297,257 | $301,134 | 22 | <$5M | AI Deep Dive |
| Jamestown Optometric Group, PC | Health Care and Social Assistance | $287,500 | $290,186 | 31 | $7.3M | AI Deep Dive |
| Citadel Services, Inc. | Construction | $285,677 | $287,915 | 18 | $5.7M | AI Deep Dive |
| Mwm Motors, Inc. | Retail Trade | $280,400 | $283,539 | 32 | $8.1M | AI Deep Dive |
| Anthony J Peppy DDS & Samuel J Peppy Jr DDS PC | Health Care and Social Assistance | $270,520 | $271,750 | 27 | $6.3M | AI Deep Dive |
| Support Enterprises Inc. | Manufacturing | $270,000 | $271,583 | 65 | $22.9M | AI Deep Dive |
| Lane Women's Health Group, P.c. | Health Care and Social Assistance | $268,465 | $270,510 | 23 | $7.2M | AI Deep Dive |
| Naco Truck Leasing Inc. | Transportation and Warehousing | $268,082 | $271,101 | 34 | $6.9M | AI Deep Dive |
| Superior Steel Door & Trim Co INC | Manufacturing | $268,053 | $270,535 | 29 | $7.6M | AI Deep Dive |
| National Comedy Center Inc. | Arts, Entertainment, and Recreation | $264,154 | $266,202 | 35 | $5.3M | AI Deep Dive |
| Rollform of Jamestown, Inc. | Manufacturing | $262,342 | $264,053 | 20 | <$5M | AI Deep Dive |
| Jamestown Electro Plating Works Inc. | Manufacturing | $259,120 | $260,504 | 34 | $8.3M | AI Deep Dive |
| Weaver Material Service Inc. | Wholesale Trade | $258,140 | $260,523 | 11 | <$5M | AI Deep Dive |
| Citadel Services INC | Construction | $253,652 | $255,257 | 18 | $5.7M | AI Deep Dive |
| All Metal Specialties, Inc. | Manufacturing | $252,397 | $254,409 | 20 | <$5M | AI Deep Dive |
| Eminence Group, INC | Manufacturing | $249,897 | $251,287 | 31 | $8.1M | AI Deep Dive |