| Javen Construction Co., Inc. | Construction | $712,500 | $719,137 | 34 | $38.7M |  Research |
| St. Joseph's Church and School | Other Services (except Public Administration) | $652,200 | $656,928 | 38 | N/A | Research |
| Marlin Enterprises of Ny, LLC | Accommodation and Food Services | $591,100 | $596,509 | 112 | $19.9M | Research |
| Heathwood Assisted Living at Penfield, Inc. | Health Care and Social Assistance | $578,400 | $584,834 | 47 | $8.0M | Research |
| Penfield Place, LLC | Health Care and Social Assistance | $546,075 | $553,250 | 78 | $13.3M | Research |
| Ecc Technologies Inc. | Professional, Scientific, and Technical Services | $526,800 | $529,668 | 18 | <$5M | Research |
| Ecc Technologies Inc. | Professional, Scientific, and Technical Services | $526,800 | $532,858 | 20 | <$5M | Research |
| The Charles Finney School | Educational Services | $402,502 | $405,667 | 67 | N/A | Research |
| Baird Constructors LLC | Construction | $366,507 | $368,502 | 24 | $27.3M | Research |
| Midvale Golf and Country Club Inc. | Arts, Entertainment, and Recreation | $365,578 | $367,021 | 89 | N/A | Research |
| Penfield Obstetrics & Gynecology, LLP | Health Care and Social Assistance | $307,340 | $311,264 | 25 | $7.8M | Research |
| Shadow Lake Realty, LLC | Arts, Entertainment, and Recreation | $306,100 | $308,107 | 126 | $17.6M | Research |
| R-J Taylor General Contractors | Construction | $299,622 | $301,253 | 19 | $21.6M | Research |
| Penfield Pediatrics, LLC | Health Care and Social Assistance | $295,700 | $298,296 | 20 | $6.3M | Research |
| Quality Services LLC | Administrative and Support and Waste Management | $295,000 | $298,778 | 20 | <$5M | Research |
| Penfield Obstetrics & Gynecology LLP | Health Care and Social Assistance | $291,020 | $293,238 | 30 | $9.4M | Research |
| Monte Alban of Penfield INC | Accommodation and Food Services | $284,609 | $287,759 | 27 | <$5M | Research |
| Jimico Ent. Inc. | Retail Trade | $263,275 | $265,655 | 60 | $20.1M | Research |
| Jimico Enterprises Inc. | Retail Trade | $256,000 | $258,392 | 39 | $13.1M | Research |
| Nifty Bar Grinding & Cutting Solutions, Inc. | Manufacturing | $250,847 | $253,711 | 22 | $5.4M | Research |
| Mason Digital LLC | Professional, Scientific, and Technical Services | $250,500 | $275,112 | 15 | <$5M | Research |
| Dr Verrone & Dr Peters Od PC | Health Care and Social Assistance | $249,722 | $252,356 | 24 | $5.6M | Research |
| Dr Verrone and Dr. Peters, O.d., P.c. | Health Care and Social Assistance | $248,200 | $250,192 | 22 | $5.2M | Research |
| Mason Marketing LLC | Professional, Scientific, and Technical Services | $239,000 | $240,009 | 27 | <$5M | Research |
| Mason Marketing LLC | Professional, Scientific, and Technical Services | $239,000 | $240,328 | 33 | <$5M | Research |