| Lifetime Assistance Inc. | Health Care and Social Assistance | $10,000,000 | $10,128,219 | 259 | $44.1M |  Research |
| Employee Relations Associates Inc. | Administrative and Support and Waste Management | $10,000,000 | $10,114,444 | 500 | $78.7M | Research |
| Heritage Christian Services Inc. | Health Care and Social Assistance | $10,000,000 | $10,095,068 | 500 | $85.1M | Research |
| Labella and Associates Dpc | Professional, Scientific, and Technical Services | $9,846,410 | $9,963,758 | 500 | $61.7M | Research |
| Postler & Jaeckle Corp. | Construction | $9,645,400 | $9,773,829 | 369 | $116.7M | Research |
| The Pike Companies Limited Inc. | Construction | $9,129,900 | $9,238,444 | 440 | $501.3M | Research |
| Indotronix International Corporation | Professional, Scientific, and Technical Services | $6,971,400 | $7,047,698 | 500 | $103.4M | Research |
| Cpl Architects, Engineers, Landscape Architect and Surveyor | Professional, Scientific, and Technical Services | $6,762,800 | $5,466,871 | 432 | $53.3M | Research |
| Bergmann Associates,architects,engineers,landscapearchitects | Professional, Scientific, and Technical Services | $6,353,880 | $6,426,526 | 383 | $47.2M | Research |
| Young Men's Christian Association of Greater Rochester | Other Services (except Public Administration) | $5,967,610 | $5,853,219 | 500 | $95.5M | Research |
| Billitier Electric INC | Construction | $5,917,200 | $5,985,083 | 250 | $82.3M | Research |
| St. Johns Health Care Corporation | Health Care and Social Assistance | $5,893,900 | $5,958,405 | 500 | N/A | Research |
| The Dorschel Group | Retail Trade | $5,474,100 | $5,539,181 | 491 | $123.7M | Research |
| Mary Cariola Children's Center, INC | Health Care and Social Assistance | $5,159,700 | $5,243,115 | 500 | N/A | Research |
| Schuler-Haas Electric Corp. | Construction | $5,114,829 | $5,192,688 | 325 | $106.9M | Research |
| Wright Beverage Distributing | Wholesale Trade | $5,050,699 | $5,146,731 | 445 | $114.5M | Research |
| Harter Secrest & Emery | Professional, Scientific, and Technical Services | $4,939,600 | $5,002,717 | 239 | $58.1M | Research |
| Coordinated Care Services, Inc. | Other Services (except Public Administration) | $4,874,892 | $4,927,915 | 411 | N/A | Research |
| Brinkman International Group, Inc. | Manufacturing | $4,848,722 | $4,905,083 | 286 | $69.9M | Research |
| Century Mold Company, Inc. | Manufacturing | $4,610,817 | $4,663,747 | 380 | $376.9M | Research |
| Crosby-Brownlie, Inc. | Construction | $4,600,000 | $4,675,079 | 250 | $79.1M | Research |
| Reliant Staffing Systems INC | Administrative and Support and Waste Management | $4,520,330 | $4,613,461 | 32 | $5.0M | Research |
| Horizon Solutions LLC | Wholesale Trade | $4,409,800 | $4,459,818 | 256 | $78.4M | Research |
| Rochester Midland Corporation | Manufacturing | $4,404,250 | $4,454,205 | 313 | $310.4M | Research |
| Depaul Adult Care Communities Inc. | Health Care and Social Assistance | $4,323,650 | $4,351,874 | 500 | $85.1M | Research |