| Home Life Care, Inc. | Health Care and Social Assistance | $2,257,100 | $2,179,423 | 500 | $85.6M |  Research |
| Roanoke Chowan Community Health Center INC | Health Care and Social Assistance | $2,082,330 | $2,106,566 | 169 | N/A | Research |
| Integrated Family Services Pllc | Health Care and Social Assistance | $1,300,000 | $1,317,514 | 200 | $46.8M | Research |
| Medex Medical Transport Service, Inc. | Transportation and Warehousing | $1,049,200 | $1,063,452 | 162 | $49.7M | Research |
| Accordius Health at Creekside Care LLC | Health Care and Social Assistance | $916,560 | $928,337 | 100 | $17.0M | Research |
| United Home Care, INC | Health Care and Social Assistance | $682,800 | $689,457 | 268 | $45.9M | Research |
| Carolina's Home Care, Inc. | Health Care and Social Assistance | $367,375 | $370,865 | 124 | $21.2M | Research |
| Vals Home Health Care Incorporated | Health Care and Social Assistance | $270,833 | $274,373 | 100 | $17.1M | Research |
| Hertford County Educational Foundation, INC | Educational Services | $261,052 | $262,763 | 50 | N/A | Research |
| Ahoskie House LLC | Health Care and Social Assistance | $259,795 | $261,738 | 56 | $9.5M | Research |
| Ace Hardware of Ahoskie INC | Retail Trade | $212,500 | $214,554 | 55 | $11.4M | Research |
| Ahoskie House, LLC | Health Care and Social Assistance | $208,274 | $209,883 | 45 | $7.7M | Research |
| North State Provision Company INC | Wholesale Trade | $206,406 | $207,650 | 25 | $6.4M | Research |
| Strickland Auto Group | Retail Trade | $177,900 | $179,254 | 22 | $5.5M | Research |
| W.t. Jernigan & Sons Trucking, Inc. | Transportation and Warehousing | $167,759 | $169,096 | 25 | <$5M | Research |
| W T Jernigan and Sons Trucking Inc. | Transportation and Warehousing | $167,759 | $169,548 | 25 | <$5M | Research |
| Deeply Rooted Home Care Incorporated | Health Care and Social Assistance | $165,705 | $167,123 | 38 | $6.5M | Research |
| Central Ford Inc. | Retail Trade | $161,300 | $162,252 | 19 | <$5M | Research |
| Carolina Pediatrics PA | Health Care and Social Assistance | $160,700 | $161,701 | 11 | <$5M | Research |
| Edwards Eye Care Od Pllc | Health Care and Social Assistance | $158,000 | $159,870 | 5 | <$5M | Research |
| Eddie Harrells Auto & Sports Store | Retail Trade | $153,561 | $154,781 | 21 | <$5M | Research |
| Brabble Insulation INC | Construction | $150,152 | $151,345 | 29 | $9.3M | Research |
| Magette Well & Pump Company INC | Utilities | $124,100 | $125,182 | 10 | N/A | Research |
| Northeastern Orthopedics, P.a. | Health Care and Social Assistance | $119,100 | $120,288 | 15 | <$5M | Research |
| Ahoskie Body Shop INC | Other Services (except Public Administration) | $116,057 | $116,566 | 17 | <$5M | Research |