| Cherry Farms Seed Company, Inc. | Agriculture, Forestry, Fishing and Hunting | $543,400 | $547,657 | 39 | <$5M |  Research |
| Capt. Charlie's Seafood. INC | Manufacturing | $366,519 | $368,861 | 173 | $61.1M | Research |
| East Coast Al Holdings LLC | Health Care and Social Assistance | $176,287 | $177,596 | 44 | $7.5M | Research |
| East Coast Al Holdings, LLC | Health Care and Social Assistance | $170,800 | $172,232 | 39 | $6.6M | Research |
| Armstrong & Son Heating & Air, LLC | Construction | $151,220 | $153,026 | 24 | $7.6M | Research |
| Capt. Neill's Seafood INC | Manufacturing | $145,745 | $146,640 | 75 | $26.5M | Research |
| Cherry & Bateman Farms LLC | Agriculture, Forestry, Fishing and Hunting | $143,405 | $144,206 | 12 | <$5M | Research |
| Cherry & Bateman Farms LLC | Administrative and Support and Waste Management | $122,500 | $123,313 | 11 | <$5M | Research |
| Diamonite Rotary Tools of North Carolina | Manufacturing | $104,568 | $105,416 | 6 | <$5M | Research |
| Gary Comstock | Agriculture, Forestry, Fishing and Hunting | $94,398 | $94,899 | 41 | <$5M | Research |
| David Armstrong, Jr. Services, LLC | Other Services (except Public Administration) | $90,500 | $91,362 | 14 | <$5M | Research |
| Rose Welding & Crane Service, Inc. | Construction | $89,479 | $87,346 | 6 | <$5M | Research |
| Double Dee Farms, Inc. | Administrative and Support and Waste Management | $82,500 | $71,653 | 8 | <$5M | Research |
| Tyrrell County Rural Health Association | Health Care and Social Assistance | $82,457 | $82,865 | 6 | N/A | Research |
| Scuppernong Farm LLC | Agriculture, Forestry, Fishing and Hunting | $77,300 | $62,574 | 7 | <$5M | Research |
| Green Valley Farms | Agriculture, Forestry, Fishing and Hunting | $75,000 | $63,432 | 4 | <$5M | Research |
| Partnership for the Sounds, Inc. | Administrative and Support and Waste Management | $72,200 | $73,129 | 13 | N/A | Research |
| Windy Rose | Professional, Scientific, and Technical Services | $68,877 | $69,497 | 5 | <$5M | Research |
| Partnership for the Sounds Inc. | Arts, Entertainment, and Recreation | $67,277 | $67,933 | 13 | N/A | Research |
| Tyrrell County Rural Health Association | Health Care and Social Assistance | $65,800 | $66,213 | 6 | N/A | Research |
| Columbia Pharmacy, Inc. | Retail Trade | $63,332 | $63,941 | 16 | <$5M | Research |
| Windy H. Rose, Attorney at Law | Professional, Scientific, and Technical Services | $59,500 | $60,006 | 5 | <$5M | Research |
| Gary Comstock | Administrative and Support and Waste Management | $56,900 | $57,268 | 8 | <$5M | Research |
| Precise Electrical Services, Inc. | Construction | $55,537 | $55,913 | 7 | <$5M | Research |
| Sawyer & Sons Construction Inc. | Administrative and Support and Waste Management | $54,400 | $54,909 | 6 | <$5M | Research |