| Atlantic Tax Group, INC | Professional, Scientific, and Technical Services | $380,500 | $383,125 | 45 | $14.3M |  Research |
| Coastal Rehabilitation, INC | Health Care and Social Assistance | $378,900 | $381,100 | 38 | $10.3M | Research |
| Quality Sea Food Co. INC | Agriculture, Forestry, Fishing and Hunting | $367,744 | $370,952 | 58 | $9.5M | Research |
| Mid-Atlantic Christian University, INC | Educational Services | $358,800 | $361,268 | 105 | $12.6M | Research |
| Bergman Enterprises, Inc. DBA Motion Sensors, Inc. | Manufacturing | $288,705 | $290,622 | 35 | $9.2M | Research |
| Bergman Enterprises Inc. | Manufacturing | $288,705 | $290,734 | 32 | $8.4M | Research |
| G-Max Inc. | Construction | $280,700 | $282,476 | 22 | $7.0M | Research |
| The Brooks Senior Living LLC | Health Care and Social Assistance | $277,400 | $279,889 | 62 | $10.5M | Research |
| The Benjamin House, Inc. | Health Care and Social Assistance | $275,900 | $277,770 | 65 | N/A | Research |
| Atlantic Tax Group INC | Professional, Scientific, and Technical Services | $264,099 | $265,368 | 85 | $27.1M | Research |
| The Brooks Senior Living LLC | Health Care and Social Assistance | $261,100 | $263,654 | 64 | $10.9M | Research |
| Twiford Funeral Homes LLC | Other Services (except Public Administration) | $257,600 | $260,040 | 37 | $7.1M | Research |
| Northeastern Flooring INC | Retail Trade | $246,450 | $36,624 | 6 | <$5M | Research |
| Frog Island Seafood INC | Accommodation and Food Services | $237,500 | $240,383 | 28 | <$5M | Research |
| Skills INC | Health Care and Social Assistance | $231,500 | $233,590 | 72 | N/A | Research |
| Northeastern Veterinary Associates, PA | Professional, Scientific, and Technical Services | $229,460 | $231,691 | 18 | <$5M | Research |
| Jennette Brothers Inc. | Wholesale Trade | $222,688 | $223,554 | 40 | $10.3M | Research |
| Stevenson Sand, INC | Construction | $214,852 | $216,302 | 24 | $7.4M | Research |
| Jennette Brothers, INC | Wholesale Trade | $210,000 | $211,476 | 38 | $9.8M | Research |
| 3 Amigos Elizabeth City II INC | Accommodation and Food Services | $208,900 | $211,630 | 26 | <$5M | Research |
| Interstate Recovery Service N.c. Inc. | Administrative and Support and Waste Management | $205,958 | $207,119 | 16 | <$5M | Research |
| Comprehensive Rehabilitation & Pain Specialists PC | Health Care and Social Assistance | $195,959 | $198,207 | 19 | $6.0M | Research |
| The Twiford Law Firm, P.c. | Professional, Scientific, and Technical Services | $194,577 | $195,845 | 18 | <$5M | Research |
| First Investment Associates of Elizabeth City LLC | Accommodation and Food Services | $185,486 | $187,202 | 30 | $9.4M | Research |
| Quality Foods From the Sea, Inc. | Manufacturing | $184,000 | $185,610 | 36 | $12.7M | Research |