| East Carolina Anesthesia Associates Pllc | Health Care and Social Assistance | $6,772,900 | $6,305,433 | 262 | $82.2M | Research |
| Grady-White Boats, Inc. | Manufacturing | $4,606,200 | $4,665,441 | 321 | $89.6M | Research |
| Coastal Plains Restaurants, LLC | Accommodation and Food Services | $3,476,900 | $3,516,981 | 500 | $88.8M | Research |
| Coastal Agrobusiness INC | Wholesale Trade | $3,189,253 | $3,244,230 | 222 | $57.1M | Research |
| Sds Restaurant Group, LLC | Accommodation and Food Services | $3,054,600 | $3,920,992 | 500 | $88.8M | Research |
| Triest Ag Group, Inc. | Manufacturing | $2,904,100 | $2,937,517 | 206 | $204.3M | Research |
| Agl Capital LLC | Administrative and Support and Waste Management | $2,757,297 | $2,796,665 | 500 | $78.7M | Research |
| Port Health Services | Health Care and Social Assistance | $2,674,000 | $2,707,722 | 297 | $50.5M | Research |
| Carolina Donor Services | Health Care and Social Assistance | $2,172,299 | $0 | 127 | N/A | Research |
| Grover Gaming INC | Professional, Scientific, and Technical Services | $1,716,735 | $1,726,988 | 142 | $29.4M | Research |
| Garris Evans Lumber Co | Retail Trade | $1,697,300 | $1,708,851 | 162 | $33.4M | Research |
| Eastern Dermatology & Pathology, PA | Health Care and Social Assistance | $1,648,537 | $1,672,037 | 147 | $46.1M | Research |
| Kinetic Physical Therapy and Wellness | Health Care and Social Assistance | $1,586,800 | $1,595,439 | 140 | $37.8M | Research |
| Er Imaging INC | Health Care and Social Assistance | $1,536,800 | $1,545,722 | 176 | $55.2M | Research |
| Eastern Nephrology Associates, Pllc | Health Care and Social Assistance | $1,516,700 | $1,532,407 | 130 | $40.8M | Research |
| Pitt Electrical Ori LLC | Construction | $1,411,700 | $1,420,837 | 133 | $43.8M | Research |
| Agl Capag LLC | Administrative and Support and Waste Management | $1,284,935 | $1,291,895 | 280 | $44.1M | Research |
| Unx Industries, Inc. | Manufacturing | $1,237,341 | $1,255,206 | 106 | $105.1M | Research |
| Eastern Radiologists, Inc. | Health Care and Social Assistance | $1,208,300 | $1,215,718 | 58 | $18.2M | Research |
| Unx Industries Inc. | Manufacturing | $1,191,772 | $1,203,524 | 101 | $100.2M | Research |
| Laborquick Inc. | Construction | $1,183,580 | $1,195,054 | 161 | $49.9M | Research |
| North State Steel INC | Manufacturing | $1,109,915 | $1,120,613 | 72 | $17.6M | Research |
| Craig F Goess INC | Retail Trade | $1,080,400 | $1,087,483 | 70 | $17.6M | Research |
| Orthopaedics East & Sports Medicine Center INC | Health Care and Social Assistance | $1,078,600 | $1,084,652 | 96 | $30.1M | Research |
| Copypro, INC | Real Estate and Rental and Leasing | $992,100 | $997,419 | 86 | $38.9M | Research |