| Furnitureland South, Inc. | Retail Trade | $5,506,200 | $5,570,313 | 490 | $88.8M |  Research |
| Family Service of the Piedmont, Inc. | Health Care and Social Assistance | $1,326,900 | $1,343,913 | 153 | N/A | Research |
| Mgt, Inc. | Real Estate and Rental and Leasing | $740,900 | $0 | 117 | $17.6M | Research |
| The College Preparatory & Leadership Academy | Educational Services | $728,600 | $735,279 | 94 | N/A | Research |
| Top Cuts, LLC | Other Services (except Public Administration) | $442,934 | $446,163 | 84 | $16.0M | Research |
| Top Cuts, LLC | Other Services (except Public Administration) | $372,000 | $374,718 | 80 | $15.3M | Research |
| Southern Roots Restaurant Inc. | Accommodation and Food Services | $287,732 | $290,270 | 36 | $6.4M | Research |
| Champion Systems, Inc. | Construction | $274,946 | $276,466 | 32 | $10.5M | Research |
| Wynnefield Properties, Inc. | Real Estate and Rental and Leasing | $273,000 | $0 | 18 | <$5M | Research |
| Southern Roots Restaurant INC | Accommodation and Food Services | $205,523 | $206,665 | 36 | $6.4M | Research |
| Msj Glass and Glazing Inc. | Construction | $198,000 | $200,063 | 19 | <$5M | Research |
| Restoration Medical Clinics PC | Health Care and Social Assistance | $185,700 | $187,242 | 18 | $5.6M | Research |
| Full Moon Jamestown LLC | Accommodation and Food Services | $181,788 | $183,621 | 38 | $6.7M | Research |
| Absolute Comfort Heating & Cooling INC | Construction | $153,100 | $153,980 | 16 | $5.1M | Research |
| Jamestown United Methodist Church INC | Other Services (except Public Administration) | $152,100 | $153,068 | 35 | $6.7M | Research |
| Oakdale Pizza LLC | Accommodation and Food Services | $150,842 | $152,078 | 48 | $8.5M | Research |
| Mckenzie Industries, Inc. | Professional, Scientific, and Technical Services | $149,770 | $151,202 | 12 | <$5M | Research |
| Mckenzie Industries Inc. | Professional, Scientific, and Technical Services | $149,770 | $151,141 | 12 | <$5M | Research |
| Margachel Enterprises LLC | Health Care and Social Assistance | $149,000 | $150,168 | 48 | $8.2M | Research |
| Jamestown Engineering Group INC | Administrative and Support and Waste Management | $144,100 | $145,637 | 12 | <$5M | Research |
| Kca Recruiting, LLC | Administrative and Support and Waste Management | $136,720 | $128,093 | 15 | <$5M | Research |
| Margachel Enterprises LLC | Health Care and Social Assistance | $132,900 | $133,799 | 48 | $8.2M | Research |
| Preferred Childcare INC | Health Care and Social Assistance | $132,500 | $133,059 | 85 | $13.8M | Research |
| Kca Recruiting LLC | Administrative and Support and Waste Management | $126,722 | $127,531 | 13 | <$5M | Research |
| Preferred Childcare, Inc. | Health Care and Social Assistance | $125,000 | $126,132 | 246 | $39.9M | Research |