| Meherrin River Forest Products, Inc. | Agriculture, Forestry, Fishing and Hunting | $874,650 | $880,821 | 115 | $11.8M |  Research |
| Weldon Steel Corporation | Construction | $652,200 | $658,829 | 62 | $14.3M | Research |
| Roanoke Valley Steel Corporation | Construction | $513,800 | $517,558 | 43 | $9.9M | Research |
| Ralph's Barbeque, Inc. | Accommodation and Food Services | $381,227 | $384,086 | 49 | $8.7M | Research |
| Ralph's Barbeque, Inc. | Accommodation and Food Services | $272,300 | $274,418 | 56 | $9.9M | Research |
| Roanoke Cotton Company, LLC | Agriculture, Forestry, Fishing and Hunting | $245,626 | $247,905 | 12 | <$5M | Research |
| Roanoke Cotton Company LLC | Agriculture, Forestry, Fishing and Hunting | $238,949 | $241,784 | 60 | <$5M | Research |
| Concrete Services | Construction | $163,700 | $165,264 | 13 | <$5M | Research |
| Sorto Plumbing LLC | Construction | $104,165 | $20,887 | 5 | <$5M | Research |
| Helping Hands Mh Dd Services LLC | Health Care and Social Assistance | $96,878 | $98,199 | 29 | <$5M | Research |
| Roanoke Valley Adult Day Center | Health Care and Social Assistance | $85,000 | $85,569 | 15 | N/A | Research |
| Shree Bhavani LLC | Accommodation and Food Services | $84,438 | $85,029 | 13 | <$5M | Research |
| Valley Community Church | Other Services (except Public Administration) | $72,000 | $72,679 | 11 | N/A | Research |
| Roanoke Valley Adult Day Center | Health Care and Social Assistance | $70,000 | $70,706 | 11 | N/A | Research |
| One Step Child Developmental Center Corp | Health Care and Social Assistance | $69,600 | $70,388 | 12 | <$5M | Research |
| Pierce Trailer Service INC | Other Services (except Public Administration) | $68,900 | $69,298 | 9 | <$5M | Research |
| Chockoyotte Country Club INC | Arts, Entertainment, and Recreation | $39,400 | $39,771 | 15 | <$5M | Research |
| Eastern Elecrical Corp | Construction | $35,917 | $36,019 | 6 | <$5M | Research |
| Rick's Country Market | Retail Trade | $32,315 | $32,703 | 14 | <$5M | Research |
| Shree Bhavani LLC | Accommodation and Food Services | $30,562 | $60,790 | 12 | <$5M | Research |
| Monica Vaughan | Professional, Scientific, and Technical Services | $26,299 | $26,479 | 6 | <$5M | Research |
| Edmonds Income Tax Service Inc. | Professional, Scientific, and Technical Services | $22,690 | $22,848 | 13 | <$5M | Research |
| Michael Hinderliter | Manufacturing | $22,200 | $0 | 8 | <$5M | Research |
| Orchard Inn Motel, LLC | Accommodation and Food Services | $21,672 | $21,925 | 10 | <$5M | Research |
| Mason Heating Cooling & Electrical LLC | Construction | $20,833 | $20,948 | 1 | <$5M | Research |