| Toledo Clinic, Inc. | Health Care and Social Assistance | $10,000,000 | $10,126,389 | 500 | $156.9M |  Research |
| Heidtman Steel Products Inc. | Wholesale Trade | $8,867,900 | $8,990,350 | 482 | $147.7M | Research |
| Concept Rehab, Inc. | Health Care and Social Assistance | $6,414,800 | $6,488,614 | 500 | $135.0M | Research |
| Max Auto Supply Company | Other Services (except Public Administration) | $6,020,005 | $6,088,122 | 500 | $98.0M | Research |
| Yark Automotive Group, Inc. | Retail Trade | $5,729,000 | $5,799,499 | 413 | $104.1M | Research |
| Shumaker, Loop & Kendrick, LLP | Professional, Scientific, and Technical Services | $5,385,000 | $9,439,915 | 332 | $80.7M | Research |
| Dunbar Mechanical, Inc. | Construction | $5,250,000 | $5,310,168 | 252 | $79.7M | Research |
| Harbor | Health Care and Social Assistance | $4,963,200 | $5,018,407 | 385 | N/A | Research |
| Zepf Center INC | Health Care and Social Assistance | $4,953,300 | $5,008,804 | 455 | N/A | Research |
| Renhill Holdings, Inc. | Administrative and Support and Waste Management | $4,795,500 | $4,849,183 | 300 | $47.2M | Research |
| Erie Construction Mid-West, Inc. | Construction | $4,404,800 | $4,456,067 | 462 | $140.3M | Research |
| Findley INC | Professional, Scientific, and Technical Services | $4,400,000 | $4,441,678 | 238 | $24.6M | Research |
| Laibe Electric Co. | Construction | $4,178,600 | $4,227,026 | 193 | $63.5M | Research |
| The Bostwick-Braun Company | Wholesale Trade | $4,175,400 | $4,223,185 | 338 | $103.5M | Research |
| United Collection Bureau INC | Administrative and Support and Waste Management | $4,088,600 | $4,147,658 | 500 | $94.6M | Research |
| Intertec Corporation | Professional, Scientific, and Technical Services | $3,626,600 | $3,669,011 | 169 | $20.8M | Research |
| Taylor Automotive Group, LLC | Professional, Scientific, and Technical Services | $3,335,517 | $3,378,230 | 225 | $23.3M | Research |
| World Tire Corporation | Retail Trade | $3,329,855 | $3,367,778 | 311 | $84.1M | Research |
| Henry Gurtzweiler INC | Construction | $3,288,400 | $3,326,308 | 150 | $34.7M | Research |
| V M Systems Inc. | Construction | $3,098,700 | $3,136,139 | 133 | $42.1M | Research |
| The Toledo Zoological Society | Arts, Entertainment, and Recreation | $2,974,974 | $2,998,448 | 499 | N/A | Research |
| Manpower Incorporated of Toledo | Administrative and Support and Waste Management | $2,900,000 | $2,933,350 | 331 | $52.1M | Research |
| Toledo Tool and Die Company, INC | Manufacturing | $2,887,100 | $2,925,067 | 247 | $64.7M | Research |
| The Rumpf Corporation of Toledo | Administrative and Support and Waste Management | $2,866,258 | $2,929,952 | 500 | $78.7M | Research |
| Bolt Holdings LLC | Transportation and Warehousing | $2,729,400 | $2,761,167 | 199 | $40.1M | Research |