| Millcreek Community Hospital | Health Care and Social Assistance | $5,174,277 | $5,226,738 | 471 | N/A |  Research |
| Wm. T. Spaeder Co., Inc. | Construction | $5,034,000 | $5,110,406 | 215 | $68.0M | Research |
| Eriez Manufacturing Co. | Manufacturing | $4,507,100 | $4,558,431 | 330 | $86.4M | Research |
| Logistics Plus INC | Transportation and Warehousing | $3,667,400 | $3,709,500 | 269 | $104.2M | Research |
| Cmi America, Inc. | Administrative and Support and Waste Management | $3,501,600 | $3,540,409 | 121 | $22.9M | Research |
| Presbyterian Homes in the Presbytery of Lake Erie | Health Care and Social Assistance | $3,403,892 | $3,442,564 | 500 | N/A | Research |
| Erie Homes for Children & Adults | Health Care and Social Assistance | $3,391,200 | $3,430,387 | 500 | N/A | Research |
| Lakeshore Community Services, INC | Health Care and Social Assistance | $3,291,049 | $3,327,025 | 468 | N/A | Research |
| Saint Mary's Home of Erie | Retail Trade | $3,251,300 | $3,286,307 | 455 | N/A | Research |
| Stairways Behavioral Health | Health Care and Social Assistance | $3,149,800 | $0 | 267 | N/A | Research |
| Career Concepts Staffing Services, Inc. | Administrative and Support and Waste Management | $3,116,623 | $3,151,290 | 437 | $68.8M | Research |
| Lifeservice Management Corporation | Health Care and Social Assistance | $3,000,800 | $3,039,477 | 439 | $74.7M | Research |
| The Sarah A. Reed Children's Center | Health Care and Social Assistance | $2,670,442 | $2,701,390 | 322 | N/A | Research |
| Emergycare, Inc. | Health Care and Social Assistance | $2,481,845 | $2,509,927 | 284 | N/A | Research |
| Medical Associates of Erie | Health Care and Social Assistance | $2,416,720 | $2,441,491 | 204 | N/A | Research |
| Millcreek Manor | Health Care and Social Assistance | $2,411,373 | $2,435,821 | 324 | N/A | Research |
| Mcmanis Associates INC | Professional, Scientific, and Technical Services | $2,398,700 | $2,429,750 | 219 | $22.7M | Research |
| Infinity Resources, Inc. | Administrative and Support and Waste Management | $2,344,400 | $2,270,892 | 466 | $73.3M | Research |
| Achievement Center INC | Health Care and Social Assistance | $2,097,600 | $2,122,888 | 241 | N/A | Research |
| Mdw Power Systems LLC | Mining, Quarrying, and Oil and Gas Extraction | $2,085,700 | $330,304 | 18 | <$5M | Research |
| Modern Industries, Inc., | Manufacturing | $2,064,500 | $2,087,803 | 195 | $47.7M | Research |
| Modern Industries Inc. | Manufacturing | $2,000,000 | $2,023,178 | 163 | $42.7M | Research |
| Wm. T. Spaeder Co. Inc. | Construction | $2,000,000 | $2,024,877 | 193 | $61.1M | Research |
| Team Hardinger | Transportation and Warehousing | $1,883,700 | $1,900,318 | 146 | $22.1M | Research |
| Pec Management Ii, LLP | Accommodation and Food Services | $1,826,900 | $1,844,459 | 500 | $88.8M | Research |