| Chartwell Staffing Services Inc. | Administrative and Support and Waste Management | $10,000,000 | $10,111,667 | 500 | $78.7M |  Research |
| Rkl LLP | Professional, Scientific, and Technical Services | $8,752,300 | $8,854,489 | 452 | $143.9M | Research |
| Atlantic Solutions Group Inc. | Administrative and Support and Waste Management | $7,956,174 | $5,285,717 | 349 | $54.9M | Research |
| Ecore International INC | Manufacturing | $7,638,177 | $7,726,754 | 495 | $491.0M | Research |
| Wohlsen Construction Company | Construction | $7,484,900 | $7,571,184 | 389 | $443.2M | Research |
| Rettew Associates | Professional, Scientific, and Technical Services | $5,765,400 | $5,831,382 | 314 | $38.7M | Research |
| Conestoga View Nursing, L. P. | Health Care and Social Assistance | $4,801,600 | $4,856,952 | 321 | $54.6M | Research |
| Steinman Communications INC | Management of Companies and Enterprises | $4,250,200 | $4,299,195 | 425 | $69.9M | Research |
| Kalas Mfg, Inc. | Manufacturing | $4,161,600 | $4,209,921 | 352 | $92.2M | Research |
| T.w. Ponessa & Associates Counseling Services, Inc. | Health Care and Social Assistance | $3,958,999 | $3,996,390 | 495 | $155.4M | Research |
| Barley Snyder, LLP | Professional, Scientific, and Technical Services | $3,899,000 | $3,741,933 | 197 | $47.9M | Research |
| Orthopedic Associates of Lancaster Ltd | Health Care and Social Assistance | $3,859,700 | $3,904,301 | 320 | $100.4M | Research |
| High Hotels, Ltd. | Accommodation and Food Services | $3,801,248 | $3,839,682 | 254 | $79.4M | Research |
| Homesale Realty Services Group INC | Administrative and Support and Waste Management | $3,799,000 | $3,843,111 | 365 | $69.0M | Research |
| The Horst Group, Inc. | Management of Companies and Enterprises | $3,699,215 | $3,740,971 | 278 | $45.7M | Research |
| Lancaster Bible College | Educational Services | $3,556,975 | $3,418,135 | 226 | $27.1M | Research |
| Regional Gastroenterology Associates of | Health Care and Social Assistance | $3,479,358 | $3,521,587 | 177 | $55.6M | Research |
| Murray Insurance Associates INC | Finance and Insurance | $3,414,355 | $3,454,663 | 228 | $117.1M | Research |
| Robson Forensic, Inc. | Professional, Scientific, and Technical Services | $3,397,175 | $3,441,621 | 150 | $21.0M | Research |
| Kourt Security Partners DBA Select Security | Administrative and Support and Waste Management | $3,371,000 | $3,410,703 | 284 | $41.6M | Research |
| Community Action Partnership of Lancaster County Inc. | Educational Services | $3,087,480 | $1,558,448 | 103 | $13.7M | Research |
| Pai Corporation | Professional, Scientific, and Technical Services | $3,051,500 | $3,091,932 | 405 | N/A | Research |
| Clark Service Group, INC | Other Services (except Public Administration) | $2,856,307 | $2,894,182 | 202 | $43.4M | Research |
| Lancaster Orthopedic Group PC | Health Care and Social Assistance | $2,688,102 | $2,719,165 | 180 | $56.5M | Research |
| United Disabilities Services Foundation | Health Care and Social Assistance | $2,675,300 | $2,533,683 | 284 | N/A | Research |