| Ymca of Greater Brandywine | Arts, Entertainment, and Recreation | $5,055,132 | $5,124,380 | 500 | N/A |  Research |
| PA Leadership Charter School | Educational Services | $4,329,900 | $4,385,106 | 296 | N/A | Research |
| Roman Mosaic and Tile Company | Construction | $3,293,480 | $3,334,355 | 212 | $53.5M | Research |
| Children's Dental Health Associates LLC | Health Care and Social Assistance | $3,152,465 | $3,190,207 | 205 | $47.5M | Research |
| Wayne Moving & Storage Co., INC | Transportation and Warehousing | $2,366,158 | $2,394,158 | 158 | $23.9M | Research |
| Comstar Techonolgies LLC | Information | $2,308,295 | $2,342,445 | 129 | $21.7M | Research |
| Scott Honda of West Chester | Retail Trade | $2,035,600 | $2,061,254 | 135 | $34.0M | Research |
| Main Line Emergency Medicine Associates, LLC | Health Care and Social Assistance | $2,030,000 | $2,053,025 | 114 | $35.8M | Research |
| Quadratec INC | Wholesale Trade | $1,999,300 | $2,018,307 | 154 | $47.2M | Research |
| Anro, Inc. of Pennsylvania | Manufacturing | $1,997,875 | $2,020,065 | 138 | $136.9M | Research |
| Anro Inc. of Pennsylvania | Manufacturing | $1,915,272 | $1,932,113 | 102 | $101.2M | Research |
| Road-Con, Inc. | Construction | $1,883,800 | $1,904,754 | 73 | $22.6M | Research |
| Road-Con Inc. | Construction | $1,854,683 | $1,862,813 | 92 | $28.5M | Research |
| Green Lawn Fertilizing, LLC | Administrative and Support and Waste Management | $1,843,295 | $1,853,193 | 175 | $37.9M | Research |
| Mcg Utilities, INC | Construction | $1,767,478 | $1,784,281 | 104 | $32.2M | Research |
| Otto's Imported Cars, Ltd | Retail Trade | $1,754,900 | $1,766,551 | 113 | $28.5M | Research |
| E.j. Raith Mechanical, Inc. | Manufacturing | $1,752,597 | $1,762,480 | 74 | $19.4M | Research |
| Barclay Friends | Health Care and Social Assistance | $1,749,212 | $1,768,813 | 159 | N/A | Research |
| Kencor INC | Construction | $1,718,500 | $1,728,954 | 115 | $36.7M | Research |
| Barclay Friends | Health Care and Social Assistance | $1,592,822 | $1,611,630 | 153 | N/A | Research |
| Macelree Harvey Ltd | Professional, Scientific, and Technical Services | $1,533,831 | $1,544,014 | 103 | $25.0M | Research |
| Technical Professionals Group, LLC | Retail Trade | $1,500,000 | $1,511,671 | 300 | $46.8M | Research |
| Mcg Utilities, INC | Construction | $1,483,900 | $1,492,885 | 130 | $40.3M | Research |
| Aventura at Pembrooke | Health Care and Social Assistance | $1,446,640 | $1,457,651 | 94 | $16.0M | Research |
| Hunter Mechanical INC | Construction | $1,389,400 | $1,404,220 | 50 | $15.8M | Research |