| Lower Florence County Hospital District | Health Care and Social Assistance | $2,233,694 | $2,258,451 | 310 | N/A |  Research |
| Ice Recycling, LLC | Manufacturing | $760,700 | $765,744 | 76 | $75.4M | Research |
| Baker Transportation, Inc. | Transportation and Warehousing | $704,846 | $709,643 | 60 | $9.1M | Research |
| Ice Recycling, LLC | Administrative and Support and Waste Management | $695,400 | $700,944 | 76 | $14.4M | Research |
| Carolina Labor Group, INC | Other Services (except Public Administration) | $653,320 | $659,254 | 183 | $35.0M | Research |
| Carolina Prestress Corporation of Lake City | Manufacturing | $630,050 | $526,335 | 51 | $50.6M | Research |
| Healthcare Panascope, INC | Health Care and Social Assistance | $487,058 | $492,849 | 88 | $15.0M | Research |
| First Choice Ambulance INC | Health Care and Social Assistance | $467,098 | $473,765 | 69 | $16.0M | Research |
| Marshall's Marine, Inc. | Retail Trade | $458,505 | $462,022 | 56 | $14.6M | Research |
| Ltd Services, Inc. | Other Services (except Public Administration) | $445,540 | $451,171 | 99 | $18.9M | Research |
| First Choice Ambulance INC | Health Care and Social Assistance | $441,450 | $447,280 | 55 | $12.7M | Research |
| Labor Contractors, Inc. | Other Services (except Public Administration) | $340,010 | $345,649 | 109 | $20.8M | Research |
| Ddm Enterprises LLC | Agriculture, Forestry, Fishing and Hunting | $322,359 | $325,054 | 30 | <$5M | Research |
| Carbon Conversions INC | Manufacturing | $279,575 | $281,511 | 19 | $18.8M | Research |
| Expert Entertainment LLC | Construction | $278,794 | $280,409 | 32 | $10.5M | Research |
| T&m Transfer Company Inc. | Transportation and Warehousing | $274,547 | $277,216 | 21 | $5.6M | Research |
| Thomlinson & Mcwhite INC | Retail Trade | $249,995 | $251,412 | 27 | $6.5M | Research |
| Carter Manufacturing Company, Inc. | Manufacturing | $249,500 | $252,200 | 50 | $49.6M | Research |
| Lee Motors of Lake City INC | Other Services (except Public Administration) | $242,732 | $244,809 | 24 | <$5M | Research |
| The Carolina Academy | Educational Services | $222,616 | $224,044 | 61 | N/A | Research |
| Lake City Creative Alliance | Professional, Scientific, and Technical Services | $215,880 | $217,685 | 21 | N/A | Research |
| Lake City Creative Alliance | Public Administration | $215,880 | $217,739 | 18 | N/A | Research |
| Shabazz Trucking | Wholesale Trade | $208,325 | $210,060 | 10 | <$5M | Research |
| Quality Transport, Inc. | Transportation and Warehousing | $189,945 | $191,248 | 15 | <$5M | Research |
| Lake City Electric Motor Repair Ince | Manufacturing | $179,130 | $179,692 | 18 | <$5M | Research |