| Empirical Foods, Inc. | Manufacturing | $9,158,800 | $9,249,879 | 500 | $176.5M |  Research |
| American Natural Processors, Inc. | Agriculture, Forestry, Fishing and Hunting | $693,900 | $699,952 | 48 | <$5M | Research |
| Henjes, Conner & Williams, PC | Professional, Scientific, and Technical Services | $544,000 | $548,246 | 41 | $13.0M | Research |
| Heinold Hog Markets, LLC | Agriculture, Forestry, Fishing and Hunting | $450,000 | $453,488 | 53 | $8.7M | Research |
| Oral Surgery Associates P.c. | Health Care and Social Assistance | $444,800 | $447,852 | 26 | $6.0M | Research |
| Redler's Ltc Pharmacy, L.l.c. | Retail Trade | $339,700 | $385,119 | 26 | <$5M | Research |
| Blackhawk Masonry LLC | Administrative and Support and Waste Management | $304,615 | $305,901 | 35 | $7.6M | Research |
| J.b. III Foods, Inc. | Accommodation and Food Services | $262,976 | $264,189 | 147 | $26.1M | Research |
| Renal Associates, PC | Health Care and Social Assistance | $242,702 | $244,576 | 20 | $6.3M | Research |
| Hawkeye Exact | Health Care and Social Assistance | $181,137 | $182,611 | 12 | <$5M | Research |
| Larsen Logistics, LLC | Transportation and Warehousing | $151,900 | $153,248 | 13 | <$5M | Research |
| Larsen Logistics LLC | Transportation and Warehousing | $151,900 | $153,124 | 14 | <$5M | Research |
| Managed Data Services, LLC | Professional, Scientific, and Technical Services | $132,100 | $133,238 | 6 | <$5M | Research |
| Ddh LLC | Accommodation and Food Services | $127,183 | $127,894 | 18 | $5.6M | Research |
| Prodigy Learning Center & Preschool, LLC | Health Care and Social Assistance | $117,000 | $118,076 | 52 | $8.4M | Research |
| Hawkeye Exact LLC | Health Care and Social Assistance | $96,972 | $97,021 | 10 | <$5M | Research |
| Dunes Animal Hospital | Professional, Scientific, and Technical Services | $85,955 | $82,865 | 6 | <$5M | Research |
| Dakota Dunes Periodontics & Impant Dentistry LLC | Health Care and Social Assistance | $68,078 | $68,740 | 6 | <$5M | Research |
| Dakota Dunes Periodontics & Impant Dentistry LLC | Health Care and Social Assistance | $62,856 | $63,273 | 6 | <$5M | Research |
| Cwp LLC | Finance and Insurance | $36,100 | $36,352 | 5 | <$5M | Research |
| Siouxland Gastroenterology | Health Care and Social Assistance | $35,967 | $36,486 | 4 | <$5M | Research |
| Holy Cross Evangelical Lutheran Church | Other Services (except Public Administration) | $34,470 | $34,638 | 3 | <$5M | Research |
| Schipper INC | Other Services (except Public Administration) | $32,423 | $32,590 | 1 | <$5M | Research |
| Markve Brothers LLC | Finance and Insurance | $29,700 | $29,949 | 3 | <$5M | Research |
| NA Con INC | Real Estate and Rental and Leasing | $29,200 | $29,355 | 2 | <$5M | Research |