| The Jackson Clinic Professional Association | Health Care and Social Assistance | $9,122,185 | $9,223,036 | 500 | $156.9M |  Research |
| Personnel Placements Holdings LLC | Administrative and Support and Waste Management | $6,527,615 | $6,601,957 | 500 | $78.7M | Research |
| Cupples J&j Co, INC | Manufacturing | $3,285,845 | $3,323,267 | 308 | $75.3M | Research |
| Madison Haywood Developmental Services, Inc. | Health Care and Social Assistance | $2,658,507 | $2,689,523 | 500 | $81.0M | Research |
| West Tennessee Bone and Joint Clinic, PC | Health Care and Social Assistance | $2,203,200 | $2,228,476 | 205 | $64.3M | Research |
| Lane College | Educational Services | $2,059,855 | $2,083,543 | 244 | N/A | Research |
| Magic Valley Staffing Solutions Inc. | Administrative and Support and Waste Management | $1,950,000 | $1,956,892 | 332 | $52.2M | Research |
| Capitol City Residential Healthcare Tn | Health Care and Social Assistance | $1,905,806 | $1,932,853 | 463 | $91.9M | Research |
| Golden Circle Temporary Services INC | Administrative and Support and Waste Management | $1,473,451 | $1,477,165 | 301 | $47.4M | Research |
| University School of Jackson | Educational Services | $1,362,364 | $1,371,938 | 91 | N/A | Research |
| Golden Circle Ford Lincoln, Inc. | Retail Trade | $1,331,997 | $1,349,942 | 89 | $22.4M | Research |
| Rainey, Kizer, Reviere & Bell, P.l.c. | Professional, Scientific, and Technical Services | $1,324,447 | $1,333,019 | 89 | $21.6M | Research |
| Emergency Medical Care Facilities | Health Care and Social Assistance | $1,309,550 | $813,897 | 88 | $20.4M | Research |
| Hunt Services Inc. | Accommodation and Food Services | $1,239,557 | $0 | 215 | $67.2M | Research |
| Serra Jackson Automotive, LLC | Retail Trade | $1,178,400 | $1,189,183 | 98 | $24.7M | Research |
| Delta Electrical, Inc. | Construction | $1,077,428 | $1,084,072 | 112 | $36.8M | Research |
| Best One Tire of Jackson INC | Retail Trade | $1,006,100 | $1,016,189 | 94 | $25.4M | Research |
| Physicians Quality Care Pllc | Health Care and Social Assistance | $996,895 | $1,009,716 | 120 | $37.7M | Research |
| Physicians Quality Care, Pllc | Health Care and Social Assistance | $996,800 | $1,004,774 | 130 | $40.8M | Research |
| Loving Arms LLC | Health Care and Social Assistance | $993,500 | $1,002,276 | 245 | $42.0M | Research |
| Jackson Christian School | Educational Services | $905,275 | $915,610 | 149 | N/A | Research |
| Jackson Wholesale Hardware Co. | Wholesale Trade | $900,000 | $908,450 | 70 | $21.4M | Research |
| Hunt Hospitality Fayetteville Tn, LLC | Retail Trade | $892,690 | $0 | 215 | $51.4M | Research |
| Home Medical Products, Inc. | Real Estate and Rental and Leasing | $868,400 | $879,882 | 95 | $14.3M | Research |
| Central Distributors, Incorporated | Wholesale Trade | $812,200 | $652,667 | 70 | $18.0M | Research |